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Literature summary extracted from
- Asparatic acid 221 is critical in the calcium-induced modulation of the enzymatic activity of human aminopeptidase A (2007), J. Biol. Chem., 282, 37074-37081.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.7 | into the pENTR-D-TOPO vector and into the baculovirus transfer vector pDEST8 | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.11.7 | D221A | mutant constructed for the examination of Ca2+ effects on enzymatic activity | Homo sapiens |
| 3.4.11.7 | D221E | mutant constructed for the examination of Ca2+ effects on enzymatic activity | Homo sapiens |
| 3.4.11.7 | D221N | mutant constructed for the examination of Ca2+ effects on enzymatic activity | Homo sapiens |
| 3.4.11.7 | D221Q | mutant constructed for the examination of Ca2+ effects on enzymatic activity | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.7 | amastatin | - |
Homo sapiens |  |
| 3.4.11.7 | angiotensin IV | - |
Homo sapiens | |
| 3.4.11.7 | Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.7 | 0.36 | - |
kallidin | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.37 | - |
kallidin | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.5 | - |
Glu-7-amido-4-methylcoumarin | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.6 | - |
kallidin | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.84 | - |
angiotensin II | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.13 | - |
kallidin | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.18 | - |
angiotensin II | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.21 | - |
cholecystokinin-8 | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.22 | - |
angiotensin II | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.54 | - |
Glu-7-amido-4-methylcoumarin | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.65 | - |
Glu-7-amido-4-methylcoumarin | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.67 | - |
Glu-7-amido-4-methylcoumarin | mutant D221Q, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.84 | - |
Glu-7-amido-4-methylcoumarin | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.84 | - |
neurokinin B | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.95 | - |
Glu-7-amido-4-methylcoumarin | mutant D221Q, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 2.85 | - |
angiotensin II | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 2.87 | - |
Glu-7-amido-4-methylcoumarin | mutant D221E, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 2.9 | - |
cholecystokinin-8 | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 2.93 | - |
cholecystokinin-8 | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3.25 | - |
cholecystokinin-8 | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3.61 | - |
Glu-7-amido-4-methylcoumarin | mutant D221E, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3.97 | - |
neurokinin B | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 4.3 | - |
neurokinin B | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 4.31 | - |
Glu-7-amido-4-methylcoumarin | mutant D221A, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 4.36 | - |
Glu-7-amido-4-methylcoumarin | mutant D221A, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 4.94 | - |
neurokinin B | wild-type, in the absence of CaCl2 | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.7 | membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.7 | Ca2+ | - |
Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.7 | angiotensin II + H2O | Homo sapiens | angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe | aspartic acid + angiotensin III | angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.7 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.7 | using a Co2+-charged chelating Toyopearl column | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.7 | angiotensin II + H2O | angiotensin II: Asp-Arg-Val-Tyr-Ile-His-Pro-Phe | Homo sapiens | aspartic acid + angiotensin III | angiotensin III: Arg-Val-Tyr-Ile-His-Pro-Phe | ? | |
| 3.4.11.7 | cholecystokinin-8 + H2O | CCK8 | Homo sapiens | ? | - |
? | |
| 3.4.11.7 | Glu-7-amido-4-methylcoumarin + H2O | activity assay | Homo sapiens | Glu + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.11.7 | kallidin + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.11.7 | neurokinin B + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.7 | homodimer | - |
Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.7 | aminopeptidase A | - |
Homo sapiens |
| 3.4.11.7 | hAPA | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.7 | 37 | - |
activity assay | Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.7 | 0.031 | 0.51 | cholecystokinin-8 | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.031 | 0.51 | cholecystokinin-8 | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.052 | 2.1 | neurokinin B | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.052 | 2.1 | cholecystokinin-8 | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.58 | - |
Glu-7-amido-4-methylcoumarin | mutant D221A, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.7 | - |
kallidin | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.72 | - |
Glu-7-amido-4-methylcoumarin | mutant D221A, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.79 | - |
kallidin | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.88 | - |
Glu-7-amido-4-methylcoumarin | mutant D221E, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.23 | - |
Glu-7-amido-4-methylcoumarin | mutant D221E, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.33 | - |
kallidin | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 1.55 | - |
kallidin | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 2.76 | - |
angiotensin II | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 2.78 | - |
Glu-7-amido-4-methylcoumarin | mutant D221Q, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 2.96 | - |
Glu-7-amido-4-methylcoumarin | mutant D221Q, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3 | 6 | angiotensin II | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3.01 | - |
angiotensin II | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3.22 | - |
angiotensin II | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3.4 | - |
Glu-7-amido-4-methylcoumarin | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3.41 | - |
Glu-7-amido-4-methylcoumarin | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3.68 | - |
Glu-7-amido-4-methylcoumarin | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 3.88 | - |
angiotensin II | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 5.25 | - |
Glu-7-amido-4-methylcoumarin | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 7.71 | - |
cholecystokinin-8 | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 7.81 | - |
neurokinin B | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 8.22 | - |
cholecystokinin-8 | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 8.87 | - |
neurokinin B | wild-type, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 9.18 | - |
cholecystokinin-8 | wild-type, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 9.8 | - |
neurokinin B | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 9.95 | - |
cholecystokinin-8 | mutant D221N, in the presence of 1.0 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 10.47 | - |
neurokinin B | mutant D221N, in the absence of CaCl2 | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.7 | 7.5 | - |
activity assay | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.7 | 0.0000059 | - |
amastatin | mutant D221, in the presence of 1 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.0000177 | - |
amastatin | mutant D221, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.0000243 | - |
amastatin | wild-type enzyme, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.0001927 | - |
amastatin | wild-type enzyme, in the presence of 1 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.00026 | - |
angiotensin IV | mutant D221, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.000324 | - |
angiotensin IV | mutant D221, in the presence of 1 mM CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.000386 | - |
angiotensin IV | wild-type enzyme, in the absence of CaCl2 | Homo sapiens | |
| 3.4.11.7 | 0.001297 | - |
angiotensin IV | wild-type enzyme, in the presence of 1 mM CaCl2 | Homo sapiens | |
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