Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bolumar, T.; Sanz, Y.; Aristoy, M.C.; Toldra, F.
    Purification and characterisation of proteases A and D from Debaryomyces hansenii (2008), Int. J. Food Microbiol., 124, 135-141.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.37 iodoacetate 11% activation at 1 mM Debaryomyces hansenii

Application

EC Number Application Comment Organism
3.4.23.25 food industry possibly involved in ripening processes of fermented meat products Debaryomyces hansenii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.25 2-mercaptoethanol
-
 Debaryomyces hansenii
3.4.23.25 DTT
-
 Debaryomyces hansenii
3.4.23.25 HgCl2 complete inhibition at 0.05 mM Debaryomyces hansenii
3.4.23.25 iodoacetate weak inhibitor Debaryomyces hansenii
3.4.23.25 leupeptin strong inhibitor Debaryomyces hansenii
3.4.23.25 p-chloromercuribenzoic acid complete inhibition at 0.1 mM Debaryomyces hansenii
3.4.23.25 pepstatin A
-
 Debaryomyces hansenii
3.4.23.25 PMSF weak inhibitor Debaryomyces hansenii
3.4.24.37 1,10-phenanthroline complete inhibition at 0.1 mM Debaryomyces hansenii
3.4.24.37 2-mercaptoethanol 19% inhibition at 5 mM Debaryomyces hansenii
3.4.24.37 3,4-dichloroisocoumarin 71% inhibition at 1 mM Debaryomyces hansenii
3.4.24.37 4-chloromercuribenzoate complete inhibition at 0.1 mM Debaryomyces hansenii
3.4.24.37 Cd2+ 33% inhibition at 0.5 mM Debaryomyces hansenii
3.4.24.37 DTT 19% inhibition at 1 mM Debaryomyces hansenii
3.4.24.37 EGTA 35% inhibition at 5 mM Debaryomyces hansenii
3.4.24.37 Hg2+ complete inhibition at 0.05 mM Debaryomyces hansenii
3.4.24.37 Mg2+ 21% inhibition at 0.5 mM Debaryomyces hansenii
3.4.24.37 additional information no inhibition by EDTA at 5 mM Debaryomyces hansenii
3.4.24.37 pepstatin A 15% inhibition at 1 mM Debaryomyces hansenii
3.4.24.37 PMSF 50% inhibition at 1 mM Debaryomyces hansenii
3.4.24.37 Zn2+ 73% inhibition at 0.5 mM Debaryomyces hansenii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.37 additional information metalloprotease, not affeted by Cu2+, Co2+, Mn2+, and Ca2+ Debaryomyces hansenii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.25 42000
-
 1 * 42000, SDS-PAGE, native mass by gel filtration Debaryomyces hansenii
3.4.23.25 55000
-
 gel filtration Debaryomyces hansenii
3.4.24.37 65000
-
 1 * 65000, SDS-PAGE Debaryomyces hansenii
3.4.24.37 68000
-
 gel filtration Debaryomyces hansenii

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.25 Debaryomyces hansenii
-
-
-
3.4.23.25 Debaryomyces hansenii CECT12487
-
-
-
3.4.24.37 Debaryomyces hansenii
-
 isolated from the natural microflora of fermented sausages and selected as possible starter culture on the basis of its physiological and biochemical properties and its ability to compete during sausage manufacturing processes
-
3.4.24.37 Debaryomyces hansenii CECT 12487
-
 isolated from the natural microflora of fermented sausages and selected as possible starter culture on the basis of its physiological and biochemical properties and its ability to compete during sausage manufacturing processes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.25
-
 Debaryomyces hansenii
3.4.24.37 native enzyme 2560fold by fractionation with protamine sulfate, anion exchange chromatography on a weak followed by a strong anion exchange resin, and gel filtration Debaryomyces hansenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.25 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Debaryomyces hansenii N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.23.25 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Debaryomyces hansenii CECT 12487 N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.23.25 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Debaryomyces hansenii CECT12487 N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.24.37 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Debaryomyces hansenii N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.24.37 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Debaryomyces hansenii CECT 12487 N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?
3.4.24.37 N-succinyl-Leu-Tyr-7-amido-4-methylcoumarin + H2O
-
 Debaryomyces hansenii CECT12487 N-succinyl-Leu-Tyr + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.25 monomer 1 * 42000, SDS-PAGE, native mass by gel filtration Debaryomyces hansenii
3.4.24.37 monomer 1 * 65000, SDS-PAGE Debaryomyces hansenii

Synonyms

EC Number Synonyms Comment Organism
3.4.23.25 PRA
-
 Debaryomyces hansenii
3.4.23.25 Protease A aspartic protease Debaryomyces hansenii
3.4.24.37 PrD
-
 Debaryomyces hansenii
3.4.24.37 protease D
-
 Debaryomyces hansenii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.37 37
-
 assay at Debaryomyces hansenii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.25 5 6
-
 Debaryomyces hansenii
3.4.24.37 7 8
-
 Debaryomyces hansenii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.25 4 8 very low activity at other pH Debaryomyces hansenii