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Literature summary extracted from

  • Back, J.H.; Chung, J.H.; Park, J.H.; Han, Y.S.
    A versatile endonuclease IV from Thermus thermophilus has uracil-excising and 3-5 exonuclease activity (2006), Biochem. Biophys. Res. Commun., 346, 889-895.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.21.2 into the pMal-c2x vector for expression in Escherichia coli TB1 cells Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.2 EDTA in the presence of 10 mM EDTA enzymatic activity is completely eliminated Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.2 Mg2+
-
 Thermus thermophilus
3.1.21.2 Mn2+
-
 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.2 29000
-
 determined by SDS-PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.2 Thermus thermophilus Q72KH8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.2 using a amylose resin affinity column, the fusion protein is cleaved with thrombin to remove the MBP tag, the cleavage mixture is applied to a Q anion-exchange column Thermus thermophilus

Storage Stability

EC Number Storage Stability Organism
3.1.21.2 -20°C, Tris-buffer, pH 6.8, 50 mM NaCl, 0.5 mM DTT, 50% glycerol Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.21.2 endonuclease IV endonuclease IV of Thermus thermophilus possesses a 3'-5' exonuclease activity similar to that of Escherichia coli exonuclease III Thermus thermophilus
3.1.21.2 TthNfo
-
 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.2 7.5
-
 assay on the uracil-processing activity and 3'-5' exonuclease activity Thermus thermophilus
3.1.21.2 7.5
-
 DNA cleavage assay Thermus thermophilus