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Literature summary extracted from

  • Szawlowska, U.; Prus, W.; Bielawski, W.
    The molecular and biochemical characteristics of proline iminopeptidase from rye seedlings (Secale cereale L.) (2006), Acta Physiol. Plant., 28, 517-524.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.5 CuCl2 1 mM, 9% residual activity Secale cereale
3.4.11.5 diisopropyl fluorophosphate 1 mM, 49% residual activity Secale cereale
3.4.11.5 E-64 1 mM, 16% residual activity Secale cereale
3.4.11.5 additional information not inhibitory: MgCl2, MnCl2, CaCl2 Secale cereale
3.4.11.5 p-hydroxymercuribenzoic acid 1 mM, 2% residual activity Secale cereale
3.4.11.5 PbCl2 1 mM, 9% residual activity Secale cereale
3.4.11.5 phenylmethylsulfonyl fluoride 1 mM, 51% residual activity Secale cereale
3.4.11.5 ZnCl2 1 mM, 9% residual activity Secale cereale

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.5 225
-
 gel filtration Secale cereale
3.4.11.5 55400
-
 4 * 55400, SDS-PAGE Secale cereale

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.5 Secale cereale
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.5
-
 Secale cereale

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.5 seedling shoot of seedling Secale cereale
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.5 8.5
-
 pH 7.5, 37°C Secale cereale

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.5 glycyl-2-naphthylamide + H2O 6.4% of the activity with L-prolyl-2-naphthylamide Secale cereale glycine + 2-naphthylamine
-
 ?
3.4.11.5 L-alanyl-2-naphthylamide + H2O 6.1% of the activity with L-prolyl-2-naphthylamide Secale cereale L-alanine + 2-naphthylamine
-
 ?
3.4.11.5 L-arginyl-2-naphthylamide + H2O 3.2% of the activity with L-prolyl-2-naphthylamide Secale cereale L-arginine + 2-naphthylamine
-
 ?
3.4.11.5 L-histidinyl-2-naphthylamide + H2O 6.3% of the activity with L-prolyl-2-naphthylamide Secale cereale L-histidine + 2-naphthylamine
-
 ?
3.4.11.5 L-leucyl-2-naphthylamide + H2O 4.7% of the activity with L-prolyl-2-naphthylamide Secale cereale L-leucine + 2-naphthylamine
-
 ?
3.4.11.5 L-phenylalanyl-2-naphthylamide + H2O 7.6% of the activity with L-prolyl-2-naphthylamide Secale cereale L-phenylalanine + 2-naphthylamine
-
 ?
3.4.11.5 L-prolyl-2-naphthylamide + H2O
-
 Secale cereale L-proline + 2-naphthylamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.5 tetramer 4 * 55400, SDS-PAGE Secale cereale

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.5 37
-
-
 Secale cereale

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.5 37
-
 30 min, stable up to Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.5 7.8
-
-
 Secale cereale

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.5 7.5 8
-
 Secale cereale