Literature summary extracted from
Eckhard, U.; Nuess, D.; Ducka, P.; Schoenauer, E.; Brandstetter, H.
Crystallization and preliminary X-ray characterization of the catalytic domain of collagenase G from Clostridium histolyticum (2008), Acta Crystallogr. Sect. F, 64, 419-421.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.24.3 |
gene colG, expression of the catalytic domain of N-terminally His-tagged, separated from ColG by a TEV cleavage site, collagenase G in Escherichia coli strain BL21 (DE3) |
Hathewaya histolytica |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.4.24.3 |
purified recombinant His-tagged catalytic domain of collagenase G, sitting drop vapour diffusion method, 10 mg/ml protein in 25 mM Tris, pH 7.5, and 50 mM NaCl, mixed with reservoir solution containing 0.12 M sodium citrate and 23% v/v PEG 3350 at 20°C, 10 days, X-ray diffraction structure determination and anaylsis at 2.75 A resolution |
Hathewaya histolytica |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.24.3 |
Zn2+ |
the catalytic domain contains the consensus motif HEXXH that harbours the catalytic Zn2+ ion, in contrast to the zinc ligation in metzincins such as MMPs, bacterial collagenases are believed to employ glutamate as a third zinc ligand |
Hathewaya histolytica |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.24.3 |
Hathewaya histolytica |
Q9X721 |
gene colG |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.24.3 |
recombinant catalytic domain of His-tagged collagenase G from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to about 95% purity |
Hathewaya histolytica |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.24.3 |
More |
ColG possesses a single PKD-like domain and a duplicated CBD domain in addition to the catalytic domain, comprising residues Tyr119-Ala790 |
Hathewaya histolytica |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.24.3 |
ColG |
- |
Hathewaya histolytica |
3.4.24.3 |
collagenase G |
- |
Hathewaya histolytica |