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Literature summary extracted from

  • Rajapakse, S.; Ogiwara, K.; Yamano, N.; Kimura, A.; Hirata, K.; Takahashi, S.; Takahashi, T.
    Characterization of mouse tissue kallikrein 5 (2006), Zool. Sci., 23, 963-968.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B39 expressed in Escherichia coli strain BL21 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B39 antipain 13% inhibition at 0.2 mM Mus musculus
3.4.21.B39 Aprotinin 34% inhibition at 0.1 g/ml Mus musculus
3.4.21.B39 diisopropylfluorophosphate 87% inhibition at 2 mM Mus musculus
3.4.21.B39 E-64 12% inhibition at 0.5 mM Mus musculus
3.4.21.B39 leupeptin 70% inhibition at 0.1 mM Mus musculus
3.4.21.B39 additional information not inhibited by soybean trypsin inhibitor, benzamidine, pepstatin, EDTA, and chymostatin Mus musculus
3.4.21.B39 N-alpha-p-tosyl-L-leucine-chloromethylketone 14% inhibition at 0.2 mM Mus musculus
3.4.21.B39 N-alpha-p-tosyl-L-phenylalanine-chloromethylketone 14% inhibition at 0.2 mM Mus musculus
3.4.21.B39 o-phenanthroline 10% inhibition at 2 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B39 0.03
-
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
3.4.21.B39 0.03
-
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
3.4.21.B39 0.05
-
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
3.4.21.B39 0.11
-
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
3.4.21.B39 0.13
-
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B39 25500
-
non-reducing SDS-PAGE Mus musculus
3.4.21.B39 28500
-
reducing SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B39 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B39 Ni2+-chelate column chromatography and Resource Q column chromatography Mus musculus

Storage Stability

EC Number Storage Stability Organism
3.4.21.B39 -20°C, 2 months, no loss of activity Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B39 benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
Mus musculus benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
?
3.4.21.B39 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
Mus musculus benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
?
3.4.21.B39 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
Mus musculus benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
?
3.4.21.B39 collagen type IV + H2O
-
Mus musculus ?
-
?
3.4.21.B39 diglutamyl-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
Mus musculus diglutamyl-Gly-Arg + 7-amino-4-methylcoumarin
-
?
3.4.21.B39 diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
Mus musculus diglutamyl-Pro-Phe-Arg + 7-amino-4-methylcoumarin
-
?
3.4.21.B39 Fibronectin + H2O
-
Mus musculus ?
-
?
3.4.21.B39 Gelatin + H2O
-
Mus musculus ?
-
?
3.4.21.B39 high-molecular-weight kininogen + H2O
-
Mus musculus ?
-
?
3.4.21.B39 insulin-like growth factor-binding protein-3 + H2O
-
Mus musculus ?
-
?
3.4.21.B39 additional information no activity with tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, acetyl-Ile-Glu-Thr-Asp-7-amido-4-methylcoumarin, succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, casein, laminin, and single-chain tissue-type plasminogen activator Mus musculus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B39 tissue kallikrein 5 serine protease with trypsin-like activity for Arg at the P1 position Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B39 0.03
-
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
3.4.21.B39 0.03
-
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
3.4.21.B39 0.05
-
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
3.4.21.B39 0.11
-
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus
3.4.21.B39 0.13
-
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M Tris-HCl buffer (pH 8.0) Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B39 9.5
-
-
Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.B39 7 10
-
Mus musculus