Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhao, Q.; Yuan, S.; Zhang, Y.; Zhu, H.; Dai, C.; Yang, F.; Han, F.
    Expression, purification and characterization of pectate lyase A from Aspergillus nidulans in Escherichia coli (2007), World J. Microbiol. Biotechnol., 23, 1057-1064.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.22 industry food and textile industry Aspergillus nidulans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2 expression in Escherichia coli Aspergillus nidulans
4.2.2.22 overexpression in Escherichia coli Aspergillus nidulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Cu2+ 1 mM, 91% inhibition Aspergillus nidulans
4.2.2.2 EDTA 1 mM, completely represses PelA enzyme activity Aspergillus nidulans
4.2.2.2 Zn2+ 1 mM, 40% inhibition Aspergillus nidulans
4.2.2.22 Cu2+ inhibitory Aspergillus nidulans
4.2.2.22 EDTA
-
 Aspergillus nidulans
4.2.2.22 Zn2+ inhibitory Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information Km of 0.50 mg/ml for polygalacturonic acid Aspergillus nidulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ag+ 1 mM, stimulates activity up to 120% Aspergillus nidulans
4.2.2.2 Ca2+ 1 mM, activity is enhanced by 1209% Aspergillus nidulans
4.2.2.2 Fe2+ 1 mM, stimulates activity up to 592% Aspergillus nidulans
4.2.2.2 Fe3+ 1 mM, stimulates activity up to 348% Aspergillus nidulans
4.2.2.2 Mg2+ 1 mM, stimulates activity up to 368% Aspergillus nidulans
4.2.2.2 Mn2+ 1 mM, activity is enhanced by 1221% Aspergillus nidulans
4.2.2.22 Ca2+ stimulating Aspergillus nidulans
4.2.2.22 Fe2+ stimulating Aspergillus nidulans
4.2.2.22 Fe3+ stimulating Aspergillus nidulans
4.2.2.22 Mg2+ stimulating Aspergillus nidulans
4.2.2.22 Mn2+ stimulating Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 40000
-
 gel filtration Aspergillus nidulans
4.2.2.22 40000
-
 SDS-PAGE, Sephadex G-100 chromatography Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Aspergillus nidulans
-
-
-
4.2.2.22 Aspergillus nidulans
-
 GR5
-
4.2.2.22 Aspergillus nidulans GR5
-
 GR5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Aspergillus nidulans
4.2.2.22 the recombinant protein using Ni2+-nitrilotriacetateagarose column chromatography Aspergillus nidulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.22 mycelium
-
 Aspergillus nidulans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.22 6.37
-
 90% esterified pectin Aspergillus nidulans
4.2.2.22 6.38
-
 60% esterified pectin Aspergillus nidulans
4.2.2.22 57.3
-
 polygalacturonic acid Aspergillus nidulans
4.2.2.22 63.7
-
 30% esterified pectin Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 additional information PelA efficiently macerates mung bean hypocotyls and potato tuber tissues into single cells Aspergillus nidulans ?
-
 ?
4.2.2.2 additional information PelA efficiently macerates mung bean hypocotyls and potato tuber tissues into single cells Aspergillus nidulans GR5 ?
-
 ?
4.2.2.2 pectin low-esterified pectin (30%) is the optimum substrate for the PelA, higher-esterified pectin is hardly cleaved Aspergillus nidulans ?
-
 ?
4.2.2.2 pectin low-esterified pectin (30%) is the optimum substrate for the PelA, higher-esterified pectin is hardly cleaved Aspergillus nidulans GR5 ?
-
 ?
4.2.2.2 polygalacturonic acid
-
 Aspergillus nidulans ?
-
 ?
4.2.2.2 polygalacturonic acid
-
 Aspergillus nidulans GR5 ?
-
 ?
4.2.2.22 pectin low-esterified pectin is the optimum substrate for the PelA, higher-esterified pectin is hardly cleaved Aspergillus nidulans ?
-
 ?
4.2.2.22 pectin low-esterified pectin is the optimum substrate for the PelA, higher-esterified pectin is hardly cleaved Aspergillus nidulans GR5 ?
-
 ?
4.2.2.22 polygalacturonic acid
-
 Aspergillus nidulans 4,5-unsaturated polygalacturonic acid
-
 ?
4.2.2.22 polygalacturonic acid
-
 Aspergillus nidulans GR5 4,5-unsaturated polygalacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 monomer 1 * 40000, SDS-PAGE Aspergillus nidulans

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 pectate lyase A
-
 Aspergillus nidulans
4.2.2.2 PelA
-
 Aspergillus nidulans
4.2.2.22 exopectate lyase
-
 Aspergillus nidulans
4.2.2.22 pectate lyase A
-
 Aspergillus nidulans
4.2.2.22 pectate transeliminase
-
 Aspergillus nidulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 50
-
-
 Aspergillus nidulans
4.2.2.22 50
-
-
 Aspergillus nidulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 30 70 about 50% of maximal activity at 30°C and at 70°C Aspergillus nidulans
4.2.2.22 4 60
-
 Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 50
-
 half-life: 1 h Aspergillus nidulans
4.2.2.2 60
-
 readily inactivated above Aspergillus nidulans
4.2.2.22 45 55
-
 Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8 9.5
-
 Aspergillus nidulans
4.2.2.22 8.5
-
-
 Aspergillus nidulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 7.5 10 pH 7.5: about 50% of maximal activity, pH 10: about 50% of maximal activity Aspergillus nidulans
4.2.2.22 7 10
-
 Aspergillus nidulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.2 6 8 40°C, 1 h, stable Aspergillus nidulans
4.2.2.22 3 10 at 50°C Aspergillus nidulans