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Literature summary extracted from

  • Thiagarajan, S.; Jeya, M.; Gunasekaran, P.
    Purification and characterization of a high molecular weight endoxylanase from the solid-state culture of an alkali-tolerant Aspergillus fumigatus MKU1 (2006), World J. Microbiol. Biotechnol., 22, 487-492.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 iodoacetamide
-
 Aspergillus fumigatus
3.2.1.8 L-cysteine
-
 Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 beta-mercaptoethanol 22% inhibition at 10 mM Aspergillus fumigatus
3.2.1.8 diethyl dicarbonate 22% inhibition at 10 mM Aspergillus fumigatus
3.2.1.8 dithiothreitol 21% inhibition at 10 mM Aspergillus fumigatus
3.2.1.8 EDTA 19% inhibition at 10 mM Aspergillus fumigatus
3.2.1.8 Hg2+ 96% inhibition at 10 mM Aspergillus fumigatus
3.2.1.8 additional information Ce2+, Li2+, NH4+ and Mg2+ have no significant effect on xylanase activity at 10 mM Aspergillus fumigatus
3.2.1.8 N-bromosuccinimide complete inhibition at 5 mM Aspergillus fumigatus
3.2.1.8 Ni2+ complete inhibition at 10 mM Aspergillus fumigatus
3.2.1.8 SDS strong inhibition Aspergillus fumigatus
3.2.1.8 Zn2+ 81% inhibition at 10 mM Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 66000
-
 SDS-PAGE Aspergillus fumigatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Aspergillus fumigatus
-
-
-
3.2.1.8 Aspergillus fumigatus MKU1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 electroelution Aspergillus fumigatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 16
-
 crude extract Aspergillus fumigatus
3.2.1.8 75
-
 after 4.7fold purification Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 beechwood xylan + H2O lower activity as compared to oat spelt xylan Aspergillus fumigatus xylo-oligosaccharides
-
 ?
3.2.1.8 beechwood xylan + H2O lower activity as compared to oat spelt xylan Aspergillus fumigatus MKU1 xylo-oligosaccharides
-
 ?
3.2.1.8 birchwood xylan + H2O lower activity as compared to oat spelt xylan Aspergillus fumigatus xylo-oligosaccharides
-
 ?
3.2.1.8 birchwood xylan + H2O lower activity as compared to oat spelt xylan Aspergillus fumigatus MKU1 xylo-oligosaccharides
-
 ?
3.2.1.8 additional information negligible activity is observed on carboxymethylcellulose Aspergillus fumigatus ?
-
 ?
3.2.1.8 additional information negligible activity is observed on carboxymethylcellulose Aspergillus fumigatus MKU1 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Aspergillus fumigatus xylo-oligosaccharides
-
 ?
3.2.1.8 oat spelt xylan + H2O
-
 Aspergillus fumigatus MKU1 xylo-oligosaccharides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 XylF2
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 90
-
-
 Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 30 70
-
 Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 110
-
 60% of XylF2 activity is retained at 110°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
-
 Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 3 9 about 47 and 41% activity is observed at pH 8 and 9, respectively Aspergillus fumigatus