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Literature summary extracted from

  • Hsu, C.; Yu, R.; Chou, C.
    Purification and characterization of a sodium-stimulated ?-galactosidasefrom Bifidobacterium longum CCRC 15708 (2006), World J. Microbiol. Biotechnol., 22, 355-361.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ca2+
-
 Bifidobacterium longum
3.2.1.23 Co2+
-
 Bifidobacterium longum
3.2.1.23 Cu2+
-
 Bifidobacterium longum
3.2.1.23 D-fructose
-
 Bifidobacterium longum
3.2.1.23 Fe2+
-
 Bifidobacterium longum
3.2.1.23 Fe3+
-
 Bifidobacterium longum
3.2.1.23 lactose competitive Bifidobacterium longum
3.2.1.23 Mg2+
-
 Bifidobacterium longum
3.2.1.23 Mn2+
-
 Bifidobacterium longum
3.2.1.23 additional information poor inhibition by D-glucose, D-galactose, maltose, or raffinose Bifidobacterium longum
3.2.1.23 Zn2+
-
 Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.85
-
 2-nitrophenyl-beta-D-galactopyranoside pH 7.0, 50°C Bifidobacterium longum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ 10fold activation Bifidobacterium longum
3.2.1.23 Na+ 10fold activation Bifidobacterium longum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 357000
-
 native PAGE Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Bifidobacterium longum
-
-
-
3.2.1.23 Bifidobacterium longum CCRC 15708
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 native enzyme 15.7fold by anion exchange chromatography and gel filtration Bifidobacterium longum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 168.6
-
 purified enzyme Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium longum 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Bifidobacterium longum CCRC 15708 2-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50
-
-
 Bifidobacterium longum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 40
-
 10 min, purified enzyme, stable up to Bifidobacterium longum
3.2.1.23 60
-
 10 min, purified enzyme, inactivation Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7
-
-
 Bifidobacterium longum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.23 6.5 7 purified enzyme, stable Bifidobacterium longum