Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dong, X.; Bessho, Y.; Shibata, R.; Nishimoto, M.; Shirouzu, M.; Kuramitsu, S.; Yokoyama, S.
    Crystal structure of tRNA pseudouridine synthase TruA from Thermus thermophilus HB8 (2006), RNA Biol., 3, 115-122.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.12
-
 Thermus thermophilus
5.4.99.12 expression in Escherichia coli Thermus thermophilus HB8

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.99.12 hanging-drop vapor diffusion method at 293 K. The crystals have a stick-like shape, and belong to the space group P4(1)2(1)2, with unit cell dimensions of a = b = 91.5 A and c = 164.0 A. Crystal structure is determined at 2.25 A resolution Thermus thermophilus
5.4.99.12 to 2.25 A resolution. Structure reveals the remarkably flexible structural features in the tRNA-binding cleft, which may be responsible for the primary tRNA interaction. The charged residues occupying the intermediate positions in the cleft may lead the tRNA to the active site for catalysis. The tRNA probably makes the melting base pairs move into the cleft, and a conformational change of the substrate tRNA may be necessary to facilitate access to the active site aspartate residue, deep within the cleft Thermus thermophilus HB8

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.12 tRNA uridine38-40 Thermus thermophilus
-
 tRNA pseudouridine38-40
-
 ?
5.4.99.12 tRNA uridine38-40 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 tRNA pseudouridine38-40
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.12 Thermus thermophilus
-
-
-
5.4.99.12 Thermus thermophilus HB8
-
-
-
5.4.99.12 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.12
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.12 tRNA uridine38-40
-
 Thermus thermophilus tRNA pseudouridine38-40
-
 ?
5.4.99.12 tRNA uridine38-40
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 tRNA pseudouridine38-40
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.12 homodimer homodimeric structure in crystals Thermus thermophilus