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Literature summary extracted from

  • Simanshu, D.K.; Savithri, H.S.; Murthy, M.R.
    Crystal structures of Salmonella typhimurium propionate kinase and its complex with Ap(4)A: Evidence for a novel Ap(4)A synthetic activity (2007), Proteins, 70, 1379-1388.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.15 cloned in pRSET C vector and overexpressed in Escherichia coli BL21(DE3)pLysS along with an N-terminal hexahistidine tag Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.15 by the hanging drop method, crystals of native TdcD (2.6 A resolution), TdcD-Ap4A complex in the presence of ATP (2.0 A resolution), and TdcD-Ap4A (2.4 A resolution), Ap4A is bound at the active site pocket, Ap4A is present in an extended conformation with one adenosine moiety present in the nucleotide binding site and other in the propionate binding site Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.15 Mg2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.15 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.15 to homogeneity by Ni-NTA affinity column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.15 additional information TdcD has Ap4A synthetic activity in the presence of ATP Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.15 TdcD
-
 Salmonella enterica subsp. enterica serovar Typhimurium