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Literature summary extracted from

  • Chen, L.; Zhou, X.; Fan, W.; Zhang, Y.
    Expression, purification and characterization of a recombinant Lipomyces starkey dextranase in Pichia pastoris (2008), Protein Expr. Purif., 58, 87-93.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.11 into vector pPIC9k-His6 and expressed in Pichia pastoris GS115 strain under the control of AOX1 promoter Lipomyces starkeyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.11 Al3+ 1 mM completely inhibits enzyme activity Lipomyces starkeyi
3.2.1.11 Cu2+ 1 mM completely inhibits enzyme activity Lipomyces starkeyi
3.2.1.11 Fe3+ 1 mM completely inhibits enzyme activity Lipomyces starkeyi
3.2.1.11 SDS 1 mM completely inhibits enzyme activity Lipomyces starkeyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.11 Ca2+ 1 mM stimulates 2fold Lipomyces starkeyi
3.2.1.11 Co2+
-
 Lipomyces starkeyi
3.2.1.11 K+
-
 Lipomyces starkeyi
3.2.1.11 Mg2+ 1 mM enhances enzyme activity by 145% Lipomyces starkeyi
3.2.1.11 Mn2+
-
 Lipomyces starkeyi
3.2.1.11 Na+
-
 Lipomyces starkeyi
3.2.1.11 Ni2+
-
 Lipomyces starkeyi
3.2.1.11 Zn2+
-
 Lipomyces starkeyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.11 66000
-
 enzyme after deglycosylation, SDS-PAGE and Western blot analysis Lipomyces starkeyi
3.2.1.11 70000
-
 SDS-PAGE and Western blot analysis Lipomyces starkeyi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.11 Lipomyces starkeyi
-
-
-
3.2.1.11 Lipomyces starkeyi 1390
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.11 17.56fold by cation-exchange chromatography, 18.18fold followed by Ni-affinity chromatography Lipomyces starkeyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.11 10.36
-
-
 Lipomyces starkeyi
3.2.1.11 182
-
 enzyme purified 17.56fold Lipomyces starkeyi
3.2.1.11 188.3
-
 enzyme purified 18.18fold Lipomyces starkeyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.11 dextran + H2O
-
 Lipomyces starkeyi ?
-
 ?
3.2.1.11 dextran + H2O
-
 Lipomyces starkeyi 1390 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.11 monomer 1 * 70000, purified enzyme, SDS-PAGE and Western blot analysis Lipomyces starkeyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.11 30
-
-
 Lipomyces starkeyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.11 4.5
-
-
 Lipomyces starkeyi