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Literature summary extracted from
- High-level constitutive expression in Pichia pastoris and one-step purification of phospholipase D from cowpea (Vigna unguiculata L. Walp) (2007), Protein Expr. Purif., 51, 162-169.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | synthesis | heterologous high-yield expression of enzyme in Pichia pastoris and purification by single calcium-dependent octyl-sepharose chromatography step gives 8 mg of pure recombinant protein from 1 l of yeast culture without proteolytic degradation of the N-terminal calcium-dependent C2 lipid binding domain | Vigna unguiculata |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | high-yield expression in Pichia pastoris | Vigna unguiculata |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 88110 | - |
x * 90000, SDS-PAGE, x * 88110, mass spectrometry | Vigna unguiculata |
| 3.1.4.4 | 90000 | - |
x * 90000, SDS-PAGE, x * 88110, mass spectrometry | Vigna unguiculata |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Vigna unguiculata | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | purification by single calcium-dependent octyl-sepharose chromatography step | Vigna unguiculata |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 27 | - |
pH 8.0, 37°C | Vigna unguiculata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Vigna unguiculata | 1,2-diacylglycerophosphate + choline | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | ? | x * 90000, SDS-PAGE, x * 88110, mass spectrometry | Vigna unguiculata |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.4.4 | Vigna unguiculata | isoelectric focusing | - |
4.7 |
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Release 2023.1 (January 2023)
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