Any feedback?
Literature summary extracted from
- Expression, purification, and characterization of His-tagged Staphylococcus xylosus lipase wild-type and its mutant Asp 290 Ala (2006), Protein Expr. Purif., 47, 516-523.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | sodium deoxycholate | - |
Staphylococcus xylosus |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) | Staphylococcus xylosus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | D290A | site-directed mutagenesis, the mutant shows reduced activity with tributyrin substrate compared to the wild-type enzyme, the mutation does not influence calcium requirement | Staphylococcus xylosus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | EDTA | - |
Staphylococcus xylosus | |
| 3.1.1.3 | EGTA | - |
Staphylococcus xylosus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.3 | additional information | - |
additional information | kinetics, recombinant His-tagged wild-type and mutant enzymes | Staphylococcus xylosus | |
| 3.1.1.3 | 20.4 | - |
tributyrin | pH 8.5, 37°C, recombinant His-tagged wild-type enzyme | Staphylococcus xylosus | |
| 3.1.1.3 | 31.25 | - |
tributyrin | pH 8.5, 37°C, native wild-type enzyme | Staphylococcus xylosus | |
| 3.1.1.3 | 34.5 | - |
tributyrin | pH 8.5, 37°C, recombinant His-tagged mutant D290A | Staphylococcus xylosus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.3 | extracellular | - |
Staphylococcus xylosus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.3 | Ca2+ | activates, the enzyme is not dependent on Ca2+ | Staphylococcus xylosus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 45000 | - |
x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Staphylococcus xylosus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Staphylococcus xylosus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | recombinant His-tagged wild-type and mutant enzymes 18fold and 8.8fold, respectively, from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography to homogeneity | Staphylococcus xylosus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 850 | - |
purified recombinant His-tagged wild-type enzyme, substrate olive oil | Staphylococcus xylosus |
| 3.1.1.3 | 1000 | - |
purified recombinant His-tagged mutant D290A, substrate tributyrin or olive oil | Staphylococcus xylosus |
| 3.1.1.3 | 1500 | - |
purified recombinant His-tagged wild-type enzyme, substrate tributyrin | Staphylococcus xylosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | additional information | residue Asp290 is important for the chain length specificity and catalytic efficiency of the enzyme | Staphylococcus xylosus | ? | - |
? | |
| 3.1.1.3 | olive oil + H2O | - |
Staphylococcus xylosus | ? | - |
? | |
| 3.1.1.3 | tributyrin + H2O | - |
Staphylococcus xylosus | dibutyrin + butyrate | - |
? | |
| 3.1.1.3 | trioctanoate + H2O | - |
Staphylococcus xylosus | dioctanoate + octanoate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | ? | x * 45000, recombinant His-tagged enzyme, SDS-PAGE | Staphylococcus xylosus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | lipase | - |
Staphylococcus xylosus |
| 3.1.1.3 | SXL | - |
Staphylococcus xylosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 37 | - |
assay at | Staphylococcus xylosus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.3 | 1200 | - |
tributyrin | pH 8.5, 37°C, recombinant His-tagged mutant D290A | Staphylococcus xylosus | |
| 3.1.1.3 | 1500 | - |
tributyrin | pH 8.5, 37°C, recombinant His-tagged wild-type enzyme | Staphylococcus xylosus | |
| 3.1.1.3 | 1900 | - |
tributyrin | pH 8.5, 37°C, native wild-type enzyme | Staphylococcus xylosus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 8.5 | - |
assay at | Staphylococcus xylosus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
