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Literature summary extracted from

  • Kanwar, S.S.; Ghazi, I.A.; Chimni, S.S.; Joshi, G.K.; Rao, G.V.; Kaushal, R.K.; Gupta, R.; Punj, V.
    Purification and properties of a novel extracellular thermotolerant metallolipase of Bacillus coagulans MTCC-6375 isolate (2006), Protein Expr. Purif., 46, 421-428.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Co2+
-
 Weizmannia coagulans
3.1.1.3 EDTA reversible by Hg2+ and Al3+ Weizmannia coagulans
3.1.1.3 Na-molybdate inhibits slightly at 1 mM Weizmannia coagulans
3.1.1.3 PMSF 98% nhibition at 15 mM Weizmannia coagulans
3.1.1.3 Zn2+
-
 Weizmannia coagulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 28
-
 4-nitrophenyl palmitate pH 8.5, 45°C Weizmannia coagulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Weizmannia coagulans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Al3+ activates at 1 mM Weizmannia coagulans
3.1.1.3 Ca2+ activates at 1 mM Weizmannia coagulans
3.1.1.3 Fe3+ activates at 1 mM Weizmannia coagulans
3.1.1.3 Hg2+ activates at 1 mM Weizmannia coagulans
3.1.1.3 Mg2+ activates at 1 mM Weizmannia coagulans
3.1.1.3 additional information enzyme activity is not affected by Na+, K+, and Cu2+ at 1-10 mM Weizmannia coagulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 103000
-
 x * 103000, SDS-PAGE Weizmannia coagulans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Weizmannia coagulans
-
-
-
3.1.1.3 Weizmannia coagulans MTCC-6375
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native extracellular enzyme 76.4fold by anion exchange and hydrophobic interaction chromatography to homogeneity Weizmannia coagulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 1.98
-
 purified enzyme, substrate 4-nitrophenyl palmitate Weizmannia coagulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl octanoate + H2O
-
 Weizmannia coagulans 4-nitrophenol + octanoate
-
 ?
3.1.1.3 4-nitrophenyl octanoate + H2O
-
 Weizmannia coagulans MTCC-6375 4-nitrophenol + octanoate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Weizmannia coagulans 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Weizmannia coagulans MTCC-6375 4-nitrophenol + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 103000, SDS-PAGE Weizmannia coagulans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 extra-cellular metallolipase
-
 Weizmannia coagulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 45
-
-
 Weizmannia coagulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 25
-
 purified enzyme, 20 min, 25% remaining activity Weizmannia coagulans
3.1.1.3 35
-
 purified enzyme, 20 min, 63% remaining activity Weizmannia coagulans
3.1.1.3 45
-
 purified enzyme, 20 min, complete stable Weizmannia coagulans
3.1.1.3 55
-
 purified enzyme, 20 min, 79% remaining activity Weizmannia coagulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.5
-
-
 Weizmannia coagulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 additional information
-
-
 Weizmannia coagulans