EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.20 | Ag+ | complete inhibition at 1 mM | Thermochaetoides thermophila | |
3.2.1.20 | Al3+ | 35% inhibition at 1 mM | Thermochaetoides thermophila | |
3.2.1.20 | Co2+ | 51% inhibition at 1 mM | Thermochaetoides thermophila | |
3.2.1.20 | Cu2+ | 46% inhibition at 1 mM | Thermochaetoides thermophila | |
3.2.1.20 | Hg2+ | complete inhibition at 1 mM | Thermochaetoides thermophila | |
3.2.1.20 | trehalose | inhibits both glycosyl hydrolase and transglycosylase activities decreasing Vmax by 3.9-4.8fold | Thermochaetoides thermophila | |
3.2.1.20 | Zn2+ | 70% inhibition at 1 mM | Thermochaetoides thermophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | additional information | - |
additional information | 4.35 mg/ml for starch | Thermochaetoides thermophila | |
3.2.1.20 | 0.55 | - |
maltose | pH 7.0, 60°C | Thermochaetoides thermophila |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.20 | additional information | the enzyme is not affected by 1 mM of Mg2+, Ca2+, Ba2+, K+, Fe2+, NH4+, Mn2+, EDTA, and 2-mercaptoethanol | Thermochaetoides thermophila |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 22000 | - |
2 * 22000 + 2 * 26000, SDS-PAGE | Thermochaetoides thermophila |
3.2.1.20 | 26000 | - |
2 * 22000 + 2 * 26000, SDS-PAGE | Thermochaetoides thermophila |
3.2.1.20 | 107000 | - |
gel filtration | Thermochaetoides thermophila |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.20 | Thermochaetoides thermophila | - |
var. coprophilum, filamentous fungus, strain ATCC 1645 | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.20 | glycoprotein | 14% carbohydrate content | Thermochaetoides thermophila |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.20 | native enzyme 64fold by anion exchange chromatography and two steps of hydrophobic interaction chromatography, to homogeneity | Thermochaetoides thermophila |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.20 | additional information | highest enzyme activity in the cells after growth on maltose or starch, effects of sugars as carbon sources on enzyme activity, overview | Thermochaetoides thermophila | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 33.33 | - |
purified enzyme | Thermochaetoides thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | amylose + H2O | high activity | Thermochaetoides thermophila | alpha-D-glucose | - |
? | |
3.2.1.20 | isomaltose + H2O | low activity | Thermochaetoides thermophila | 2 alpha-D-glucose | - |
? | |
3.2.1.20 | maltooligosaccharides + H2O | - |
Thermochaetoides thermophila | alpha-D-glucose | - |
? | |
3.2.1.20 | maltose + H2O | preferred substrate | Thermochaetoides thermophila | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | additional information | substrate specificity, overview, no activity with trehalose, the enzyme exhibits transglycosylation activity producing trehalose and maltooligosaccharides from 5% maltose, at early stages of the transglycosylation reaction trehalose is the predominant product, while at later stages maltooligosaccharides are also accumulated | Thermochaetoides thermophila | ? | - |
? | |
3.2.1.20 | starch + H2O | - |
Thermochaetoides thermophila | alpha-D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.20 | tetramer | 2 * 22000 + 2 * 26000, SDS-PAGE | Thermochaetoides thermophila |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 60 | - |
- |
Thermochaetoides thermophila |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 35 | 70 | 10% of maximal activity at 35°C, 33% at 70°C, steady increase in activity from 35 to 60°C, sharp drop of activity above 65°C | Thermochaetoides thermophila |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 7 | - |
- |
Thermochaetoides thermophila |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 4 | 7.5 | over 50% of maximal activity within this range, inactive at pH 4.0 and pH 8.0 | Thermochaetoides thermophila |