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Literature summary extracted from

  • Giannesi, G.C.; Polizeli, M.de L.; Terenzi, H.F.; Jorge, J.A.
    A novel alpha-glucosidase from Chaetomium thermophilum var. coprophilum that converts maltose into trehalose: Purification and partial characterisation of the enzyme (2006), Process Biochem., 41, 1729-1735.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Ag+ complete inhibition at 1 mM Thermochaetoides thermophila
3.2.1.20 Al3+ 35% inhibition at 1 mM Thermochaetoides thermophila
3.2.1.20 Co2+ 51% inhibition at 1 mM Thermochaetoides thermophila
3.2.1.20 Cu2+ 46% inhibition at 1 mM Thermochaetoides thermophila
3.2.1.20 Hg2+ complete inhibition at 1 mM Thermochaetoides thermophila
3.2.1.20 trehalose inhibits both glycosyl hydrolase and transglycosylase activities decreasing Vmax by 3.9-4.8fold Thermochaetoides thermophila
3.2.1.20 Zn2+ 70% inhibition at 1 mM Thermochaetoides thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
additional information 4.35 mg/ml for starch Thermochaetoides thermophila
3.2.1.20 0.55
-
maltose pH 7.0, 60°C Thermochaetoides thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 additional information the enzyme is not affected by 1 mM of Mg2+, Ca2+, Ba2+, K+, Fe2+, NH4+, Mn2+, EDTA, and 2-mercaptoethanol Thermochaetoides thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 22000
-
2 * 22000 + 2 * 26000, SDS-PAGE Thermochaetoides thermophila
3.2.1.20 26000
-
2 * 22000 + 2 * 26000, SDS-PAGE Thermochaetoides thermophila
3.2.1.20 107000
-
gel filtration Thermochaetoides thermophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Thermochaetoides thermophila
-
var. coprophilum, filamentous fungus, strain ATCC 1645
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.20 glycoprotein 14% carbohydrate content Thermochaetoides thermophila

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 native enzyme 64fold by anion exchange chromatography and two steps of hydrophobic interaction chromatography, to homogeneity Thermochaetoides thermophila

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 additional information highest enzyme activity in the cells after growth on maltose or starch, effects of sugars as carbon sources on enzyme activity, overview Thermochaetoides thermophila
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 33.33
-
purified enzyme Thermochaetoides thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 amylose + H2O high activity Thermochaetoides thermophila alpha-D-glucose
-
?
3.2.1.20 isomaltose + H2O low activity Thermochaetoides thermophila 2 alpha-D-glucose
-
?
3.2.1.20 maltooligosaccharides + H2O
-
Thermochaetoides thermophila alpha-D-glucose
-
?
3.2.1.20 maltose + H2O preferred substrate Thermochaetoides thermophila alpha-D-glucose + D-glucose
-
?
3.2.1.20 additional information substrate specificity, overview, no activity with trehalose, the enzyme exhibits transglycosylation activity producing trehalose and maltooligosaccharides from 5% maltose, at early stages of the transglycosylation reaction trehalose is the predominant product, while at later stages maltooligosaccharides are also accumulated Thermochaetoides thermophila ?
-
?
3.2.1.20 starch + H2O
-
Thermochaetoides thermophila alpha-D-glucose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 tetramer 2 * 22000 + 2 * 26000, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 60
-
-
Thermochaetoides thermophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 35 70 10% of maximal activity at 35°C, 33% at 70°C, steady increase in activity from 35 to 60°C, sharp drop of activity above 65°C Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 7
-
-
Thermochaetoides thermophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 4 7.5 over 50% of maximal activity within this range, inactive at pH 4.0 and pH 8.0 Thermochaetoides thermophila