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Literature summary extracted from

  • Ni, X.; Hager, L.P
    Expression of Batis maritima methyl chloride transferase in Escherichia coli (1999), Proc. Natl. Acad. Sci. USA, 96, 3611-3615.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.165 Urea activity in 1, 2, and 3 M urea is about 1.75-, 2-, and 1.5-fold higher than in the absence of urea, respectively Batis maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.165 expression in Escherichia coli Batis maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.165 chloride competitive inhibition of methyl iodide formation Batis maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.165 0.0294
-
 S-adenosyl-L-methionine pH 6.8, 22°C, native enzyme Batis maritima
2.1.1.165 0.23
-
 S-adenosyl-L-methionine pH 6.8, 22°C, recombinant enzyme Batis maritima
2.1.1.165 6.5
-
 Iodide pH 6.8, 22°C, recombinant enzyme Batis maritima
2.1.1.165 8.5
-
 Iodide pH 6.8, 22°C, native enzyme Batis maritima
2.1.1.165 18.5
-
 bromide pH 6.8, 22°C, native enzyme Batis maritima
2.1.1.165 25
-
 bromide pH 6.8, 22°C, recombinant enzyme Batis maritima
2.1.1.165 100
-
 chloride pH 6.8, 22°C, recombinant enzyme Batis maritima
2.1.1.165 155
-
 chloride pH 6.8, 22°C, native enzyme Batis maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.165 ammonium sulfate the activity of the recombinant methylase in 0.5 M and 1.0 M ammonium sulfate is about 1.25fold higher than in the absence of ammonium sulfate Batis maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.165 S-adenosyl-L-methionine + chloride Batis maritima this enzyme possibly functions in the control and regulation of the internal concentration of chloride ions in halophytic plant cells S-adenosyl-L-homocysteine + methyl chloride
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.165 Batis maritima Q9ZSZ7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.165 S-adenosyl-L-methionine + bromide
-
 Batis maritima S-adenosyl-L-homocysteine + methyl bromide
-
 ?
2.1.1.165 S-adenosyl-L-methionine + chloride
-
 Batis maritima S-adenosyl-L-homocysteine + methyl chloride
-
 ?
2.1.1.165 S-adenosyl-L-methionine + chloride this enzyme possibly functions in the control and regulation of the internal concentration of chloride ions in halophytic plant cells Batis maritima S-adenosyl-L-homocysteine + methyl chloride
-
 ?
2.1.1.165 S-adenosyl-L-methionine + iodide
-
 Batis maritima S-adenosyl-L-homocysteine + methyl iodide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.165 methyl chloride transferase
-
 Batis maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.165 6.2
-
 methyl bromide production Batis maritima
2.1.1.165 6.2 6.8 methyl chloride production Batis maritima
2.1.1.165 6.8 7.5 methyl iodide production Batis maritima