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Literature summary extracted from

  • Merali, Z.; Mayer, M.J.; Parker, M.L.; Michael, A.J.; Smith, A.C.; Waldron, K.W.
    Metabolic diversion of the phenylpropanoid pathway causes cell wall and morphological changes in transgenic tobacco stems (2007), Planta, 225, 1165-1178.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.61 additional information studies on genetic manipulation of cell walls in plants Nicotiana tabacum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.61 transgenic tobacco cv. XHFD 8 plants expressing various levels of bacterial HCHL, lignin deposition reduced, monolignol biosynthesis and vascular integrity impaired, reduction in stem toughness, cell wall esterified and soluble phenolics reduced, wall-bound accretion of vanillin and vanillic acid, intracellular accumulation of novel metabolites such as hydroxybenzoic and vanillic acid derivatives Nicotiana tabacum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.2.61 cell wall impact on cell wall integrity and vascular integrity, biomechanical tissue properties, carbon allocation to phenolic pools, lignin formation studied, morphological changes of the plants described Nicotiana tabacum 5618
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.61 feruloyl-CoA Nicotiana tabacum
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 4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.61 Nicotiana tabacum
-
 transgenic tobacco cv XHFD 8 expressing various levels of HCHL, the bacterial 4-hydroxycinnamoyl-CoA hydratase/lyase gene of Pseudomonas fluorescens, transgenic lines 201 and 27 reveal high expression, line 13 shows intermediate expression, pBin 19 plants serve as controls
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.61 leaf young leaves from pre- and postflowering transgenic and control plants, cell wall components analysed by HPLC, plants expressing high levels of HCHL exhibit a reduced lignin deposition, monolignol biosynthesis and vascular integrity impaired Nicotiana tabacum
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.61 additional information
-
 enzyme activity measured, quantification by integration of peaks with reference to vanillin, peak identity calculated by GC-MS and NMR Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.61 feruloyl-CoA
-
 Nicotiana tabacum 4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA
-
 ?
4.1.2.61 p-coumaroyl-CoA in transgenic plants the HCHL enzyme acts upon intermediates of the phenylpropanoid pathway Nicotiana tabacum 4-hydroxy-3-methoxy-benzaldehyde + acetyl-CoA i.e. vanillin ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.61 4-hydroxycinnamoyl-CoA hydratase/lyase
-
 Nicotiana tabacum
4.1.2.61 HCHL
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 Nicotiana tabacum