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Literature summary extracted from

  • Chantarangsee, M.; Tanthanuch, W.; Fujimura, T.; Fry, S.C.; Ketudat Cairns, J.
    Molecular characterization of ?-galactosidases from germinating rice (Oryza sativa) (2007), Plant Sci., 173, 118-134.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.23 EDTA 40% activation at 10 mM of recombinant isozyme OsGal1 Oryza sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 isozymes OsBGal1 and OsBGal2, DNA and amino acid sequence determination and anaylsis, phylogenetic tree, functional expression of soluble isozymes in Escherichia coli, expression of isozyme OsBGal1 thioredoxin fusion protein Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Cu2+ complete inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa
3.2.1.23 D-galactose 10% inhibition at 5 mM of recombinant isozyme OsGal1 Oryza sativa
3.2.1.23 D-gluconolactone 40% inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa
3.2.1.23 delta-galactonolactone 62% inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa
3.2.1.23 Fe3+ complete inhibition at 1 mM of recombinant isozyme OsGal1 Oryza sativa
3.2.1.23 Hg2+ complete inhibition at 1 mM of recombinant isozyme OsGal1 Oryza sativa
3.2.1.23 IPTG 38% inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa
3.2.1.23 lactose 15% inhibition at 5 mM of recombinant isozyme OsGal1 Oryza sativa
3.2.1.23 Mg2+ 27% inhibition at 10 mM of recombinant isozyme OsGal1 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.032
-
 4-nitrophenyl-beta-D-galactopyranoside pH 3.5, 60°C, recombinant isozyme OsGal1 Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 97000
-
 x * 97000, isozyme OsGal1, SDS-PAGE Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Oryza sativa Q10RB4 precursor; two isozymes OsBGal1 and OsBGal2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 recombinant OsBGal1 thioredoxin fusion protein 23fold from Escherichia coli by nickel affinity and anion exchange chromatography Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 internode
-
 Oryza sativa
-
3.2.1.23 leaf
-
 Oryza sativa
-
3.2.1.23 additional information developmental distribution of isozyme expression in rice tissues, overview Oryza sativa
-
3.2.1.23 panicle
-
 Oryza sativa
-
3.2.1.23 seed germinating rice, enzyme location in the embryo, aleurone layer and surrounding tissues, and in the radicle and shoot after germination Oryza sativa
-
3.2.1.23 shoot
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 0.012
-
 recombinant isozyme BGal1 in Escherichia coli, substrate 4-nitrophenyl-beta-D-galactopyranoside Oryza sativa
3.2.1.23 0.04
-
 recombinant isozyme BGal2 in Escherichia coli, substrate 2-nitrophenyl-beta-D-galactopyranoside Oryza sativa
3.2.1.23 0.058
-
 recombinant isozyme BGal1 in Escherichia coli, substrate 2-nitrophenyl-beta-D-galactopyranoside Oryza sativa
3.2.1.23 0.083
-
 recombinant isozyme BGal2 in Escherichia coli, substrate 4-nitrophenyl-beta-D-galactopyranoside Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Oryza sativa 2-nitrophenol + beta-D-galactose
-
 ir
3.2.1.23 4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Oryza sativa 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.23 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Oryza sativa 4-nitrophenol + beta-D-galactose
-
 ir
3.2.1.23 additional information the OsBGal1 isozyme releases significant amounts of galactose from 1,3-, 1,4-, and 1,6-beta-linked galactose di- and trisaccharides, with beta-1,3-galactobiose and beta-1,3-galactotriose appearing to be hydrolyzed the fastest, no activity of isozyme OsBGal1 with lactose, as well as with 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-arabinoside, and 4-nitrophenyl-beta-D-xyloside Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 ? x * 97000, isozyme OsGal1, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 More cf. EC 3.2.1.145 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 50 60 recombinant isozyme OsGal1 Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 60
-
 10 min, purified recombinant isozyme OsGal1, rapid inactivation above Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 3 3.5 recombinnat isozyme OsGal1 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 2.5 5 inactivation at pH 2.5, half-maximal activity at pH 4.5-5.0, recombinant isozyme OsGal1 Oryza sativa