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Literature summary extracted from

  • Ahn, Y.O.; Saino, H.; Mizutani, M.; Shimizu, B.; Sakata, K.
    Vicianin hydrolase is a novel cyanogenic beta-glycosidase specific to beta-vicianoside (6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside) in seeds of Vicia angustifolia (2007), Plant Cell Physiol., 48, 938-947.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.119 expressed in Escherichia coli as a GST fusion protein, full-length cDNA subcloned into the pFastBac1 plasmid for expression in insect cells using a baculovirus expression system Vicia sativa subsp. nigra

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.119 55616
-
 1 * 56000, SDS-PAGE, 1 * 55616, sequence analysis Vicia sativa subsp. nigra
3.2.1.119 56000
-
 1 * 56000, SDS-PAGE, 1 * 55616, sequence analysis Vicia sativa subsp. nigra

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.119 Vicia sativa subsp. nigra A2SY66
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.119 by gel filtration, 9.39fold Vicia sativa subsp. nigra

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.119 flower moderately present Vicia sativa subsp. nigra
-
3.2.1.119 leaf slightly present Vicia sativa subsp. nigra
-
3.2.1.119 root slightly present Vicia sativa subsp. nigra
-
3.2.1.119 seed abundantly present Vicia sativa subsp. nigra
-
3.2.1.119 stem slightly present Vicia sativa subsp. nigra
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.119 1.05
-
 crude extract Vicia sativa subsp. nigra
3.2.1.119 8.97
-
 recombinant enzyme, activity against vicianin Vicia sativa subsp. nigra
3.2.1.119 9.86
-
 native enzyme, purified, activity against vicianin Vicia sativa subsp. nigra

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.119 2-phenylethyl beta-primeveroside + H2O
-
 Vicia sativa subsp. nigra ?
-
 ?
3.2.1.119 furcatin + H2O recombinant enzyme expressed in Escherichia coli does not hydrolyze furcatin Vicia sativa subsp. nigra ?
-
 ?
3.2.1.119 additional information does not hydrolyze amygdalin, mandelonitrile-, 2-phenylethyl-, p-nitrophenyl beta-gentiobioside, p-nitrophenyl beta-D-xylopyranoside, p-nitrophenyl alpha-L-arabinopyranoside and p-nitrophenyl beta-D-galactopyranoside Vicia sativa subsp. nigra ?
-
 ?
3.2.1.119 p-nitrophenyl beta-primeveroside + H2O recombinant enzyme expressed in Escherichia coli does not hydrolyze p-nitrophenyl beta-primeveroside Vicia sativa subsp. nigra ?
-
 ?
3.2.1.119 prunasin + H2O shows 34% of its vicianin hydrolase activity with prunasin Vicia sativa subsp. nigra D-mandelonitrile + beta-D-glucose
-
 ?
3.2.1.119 vicianin + H2O best substrate, recombinant protein shows the same pattern of substrate specificity as the native enzyme Vicia sativa subsp. nigra vicianose + D-mandelonitrile
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.119 monomer 1 * 56000, SDS-PAGE, 1 * 55616, sequence analysis Vicia sativa subsp. nigra

Synonyms

EC Number Synonyms Comment Organism
3.2.1.119 vicianin hydrolase
-
 Vicia sativa subsp. nigra

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.119 Vicia sativa subsp. nigra
-
-
 8.69