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Literature summary extracted from

  • Hui, M.; Hui, K.S.
    A novel aminopeptidase with highest preference for lysine (2006), Neurochem. Res., 31, 95-102.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.6 NaF 0.4 M activates the activity by 20% Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 1,10-phenanthroline 34% residual activity at 1 mM Rattus norvegicus
3.4.11.6 actinonin
-
 Rattus norvegicus
3.4.11.6 Aprotinin 34% residual activity at 0.25 mg/ml Rattus norvegicus
3.4.11.6 arphamemine B
-
 Rattus norvegicus
3.4.11.6 bestatin highly sensitive to bestatin Rattus norvegicus
3.4.11.6 Ca2+ 19% inhibition at 1 mM Rattus norvegicus
3.4.11.6 Cd2+ complete inhibition at 1 mM Rattus norvegicus
3.4.11.6 Co2+ 76% inhibition at 1 mM Rattus norvegicus
3.4.11.6 Cu2+ complete inhibition at 1 mM Rattus norvegicus
3.4.11.6 E-64 99% residual activity at 0.1 mM Rattus norvegicus
3.4.11.6 EDTA 61% residual activity at 1 mM Rattus norvegicus
3.4.11.6 EGTA complete inhibition at 1 mM Rattus norvegicus
3.4.11.6 Hg2+ complete inhibition at 1 mM Rattus norvegicus
3.4.11.6 Leucine hydroxamate
-
 Rattus norvegicus
3.4.11.6 leucinthiol
-
 Rattus norvegicus
3.4.11.6 Mn2+ 82% inhibition at 1 mM Rattus norvegicus
3.4.11.6 additional information insensitive to L-Arg-hydroxamate, L-Lys-hydroxamate, puromycin, and amastatin Rattus norvegicus
3.4.11.6 Na+ NaCl (0.1-100 mM) has an opposite effect on the EGTA-treated KAP apo-enzyme, it inhibits 13% at 0.1 mM and 100% at 100 mM Rattus norvegicus
3.4.11.6 Ni2+ complete inhibition at 1 mM Rattus norvegicus
3.4.11.6 Pb2+ 84% inhibition at 1 mM Rattus norvegicus
3.4.11.6 pepstatin 93% residual activity at 0.1 mM Rattus norvegicus
3.4.11.6 Phenylmethanesulfonylfluoride 45% residual activity at 0.5 mM Rattus norvegicus
3.4.11.6 Zn2+ 80% inhibition at 1 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 0.333
-
 L-Lys-2-naphthylamide
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.6 cytosol
-
 Rattus norvegicus 5829
-
3.4.11.6 membrane synaptic membrane Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 K+ 0.4 M activates the activity by 55% Rattus norvegicus
3.4.11.6 Li+ 0.4 M activates the activity by 45% Rattus norvegicus
3.4.11.6 Na+ 0.4 M activates the activity by 190% Rattus norvegicus
3.4.11.6 Zn2+ Zn2+-exopeptidase Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.6 62000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 Toyopearl DEAE-650 column chromatography, Mono Q column chromatography, and DEAE-650 column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.6 adrenal gland
-
 Rattus norvegicus
-
3.4.11.6 brain
-
 Rattus norvegicus
-
3.4.11.6 cerebellum
-
 Rattus norvegicus
-
3.4.11.6 cerebral cortex
-
 Rattus norvegicus
-
3.4.11.6 hippocampus
-
 Rattus norvegicus
-
3.4.11.6 hypothalamus
-
 Rattus norvegicus
-
3.4.11.6 intestine
-
 Rattus norvegicus
-
3.4.11.6 kidney
-
 Rattus norvegicus
-
3.4.11.6 liver
-
 Rattus norvegicus
-
3.4.11.6 medulla oblongata
-
 Rattus norvegicus
-
3.4.11.6 midbrain
-
 Rattus norvegicus
-
3.4.11.6 muscle
-
 Rattus norvegicus
-
3.4.11.6 pancreas
-
 Rattus norvegicus
-
3.4.11.6 pituitary gland
-
 Rattus norvegicus
-
3.4.11.6 serum
-
 Rattus norvegicus
-
3.4.11.6 spleen
-
 Rattus norvegicus
-
3.4.11.6 testis
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 Gly-2-naphthylamide + H2O 1% activity compared to L-Lys-2-naphthylamide Rattus norvegicus Gly + 2-naphthylamine
-
 ?
3.4.11.6 Gly-L-Pro-2-naphthylamide + H2O 1% activity compared to L-Lys-2-naphthylamide Rattus norvegicus Gly-L-Pro + 2-naphthylamine
-
 ?
3.4.11.6 L-Ala-2-naphthylamide + H2O 83% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-Ala + 2-naphthylamine
-
 ?
3.4.11.6 L-Arg-2-naphthylamide + H2O 17% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-Arg + 2-naphthylamine
-
 ?
3.4.11.6 L-beta-Asn-2-naphthylamide + H2O 9% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-beta-Asn + 2-naphthylamine
-
 ?
3.4.11.6 L-Cys-2-naphthylamide + H2O 1% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-Cys + 2-naphthylamine
-
 ?
3.4.11.6 L-Gly-L-Arg-2-naphthylamide + H2O 250% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-Gly-L-Arg + 2-naphthylamine
-
 ?
3.4.11.6 L-His-2-naphthylamide + H2O 15% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-His + 2-naphthylamine
-
 ?
3.4.11.6 L-Leu-2-naphthylamide + H2O 31% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-Leu + 2-naphthylamine
-
 ?
3.4.11.6 L-Lys-2-naphthylamide + H2O 100% activity Rattus norvegicus L-Lys + 2-naphthylamine
-
 ?
3.4.11.6 L-Lys-L-Ala-2-naphthylamide + H2O 7% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-Lys-L-Ala + 2-naphthylamine
-
 ?
3.4.11.6 L-Met-2-naphthylamide + H2O 2% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-Met + 2-naphthylamine
-
 ?
3.4.11.6 L-Tyr-2-naphthylamide + H2O 62% activity compared to L-Lys-2-naphthylamide Rattus norvegicus L-Tyr + 2-naphthylamine
-
 ?
3.4.11.6 additional information no activity with L-alpha-Asn-2-naphthylamide, L-Glu-2-naphthylamide, L-Ile-2-naphthylamide, L-Phe-2-naphthylamide, L-Pro-2-naphthylamide, L-Ser-2-naphthylamide, L-Thr-2-naphthylamide, L-Trp-2-naphthylamide, L-Val-2-naphthylamide, and L-Arg-L-Arg-2-naphthylamide Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 aminopeptidase B
-
 Rattus norvegicus
3.4.11.6 APB
-
 Rattus norvegicus
3.4.11.6 KAP
-
 Rattus norvegicus
3.4.11.6 lysine-specific aminopeptidase
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.6 7.5
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.6 5 9
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.6 0.00021
-
 bestatin at 37°C in Bicine buffer complemented with 0.2 M NaCl Rattus norvegicus
3.4.11.6 0.00054
-
 leucinthiol at 37°C in Bicine buffer complemented with 0.2 M NaCl Rattus norvegicus
3.4.11.6 0.0125
-
 arphamemine B at 37°C in Bicine buffer complemented with 0.2 M NaCl Rattus norvegicus
3.4.11.6 0.02
-
 actinonin at 37°C in Bicine buffer complemented with 0.2 M NaCl Rattus norvegicus
3.4.11.6 0.024
-
 Leucine hydroxamate at 37°C in Bicine buffer complemented with 0.2 M NaCl Rattus norvegicus