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Literature summary extracted from
- Monoclonal antibodies recognize conformational epitopes on wild-type and recombinant mutant amidases from Pseudomonas aeruginosa (2007), Mol. Biotechnol., 37, 136-145.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | expression of wild-type and mutant enzymes in Escherichia coli | Pseudomonas aeruginosa |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.5.1.4 | T103I | site-directed mutagenesis, binding of specific monoclonal antibodies to the mutant enzyme compared to the wild-type enzyme | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.4 | Pseudomonas aeruginosa | - |
- |
- |
| 3.5.1.4 | Pseudomonas aeruginosa 8602 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.4 | recombinant wild-type and mutant enzymes from Escherichia coli by acetamide affinity chromatography and gel filtration | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | additional information | - |
effects of specific monoclonal antibodies on wild-type and mutant enzyme activity | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.4 | 4-nitroacetanilide + H2O | - |
Pseudomonas aeruginosa | 4-nitroaniline + acetate | - |
? |  |
| 3.5.1.4 | 4-nitroacetanilide + H2O | - |
Pseudomonas aeruginosa 8602 | 4-nitroaniline + acetate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.4 | More | epitope binding of specific monoclonal antibodies to the wild-type and T103I mutant enzyme, overview | Pseudomonas aeruginosa |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 25 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.4 | 7.2 | - |
assay at | Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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