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Literature summary extracted from

  • Alonso, G.D.; Schoijet, A.C.; Torres, H.N.; Flawia, M.M.
    TcrPDEA1, a cAMP-specific phosphodiesterase with atypical pharmacological properties from Trypanosoma cruzi (2007), Mol. Biochem. Parasitol., 152, 72-79.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.53 additional information no effector: Ca2+, calmodulin Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.53 expression in Saccharomyces cerevisiae Trypanosoma cruzi

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.53 additional information isoform PDEA1 is able to complement a yeast strain deficient in phosphodiesterase genes Trypanosoma cruzi

General Stability

EC Number General Stability Organism
3.1.4.53 Ca2+ required for stability Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.53 additional information not inhibitory: 3-isobutyl-1-methylxanthine, papaverine, theophylline; TcrPDEA1 is resistant to the typical phosphodiesterase inhibitors, such as IBMX, papaverine and theophylline Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.53 0.191
-
 adenosine 3',5'-cyclic phosphate
-
 Trypanosoma cruzi
3.1.4.53 0.191
-
 cAMP pH 7.5, 30°C Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.53 Mg2+ required Trypanosoma cruzi
3.1.4.53 Mg2+ required for activity and stability Trypanosoma cruzi
3.1.4.53 additional information no effector: Ca2+, calmodulin Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.53 Trypanosoma cruzi Q309F4
-
-
3.1.4.53 Trypanosoma cruzi Q309F4 isoform PDEA1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.53 adenosine 3',5'-cyclic phosphate + H2O no activity with cGMP Trypanosoma cruzi adenosine 5'-phosphate
-
 ?
3.1.4.53 cAMP + H2O
-
 Trypanosoma cruzi 5'-AMP
-
 ?
3.1.4.53 additional information cGMP neither activates the enzyme nor competes as a substrate Trypanosoma cruzi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.53 cAMP-specific phosphodiesterase
-
 Trypanosoma cruzi
3.1.4.53 TcrPDEA1
-
 Trypanosoma cruzi