EC Number | Application | Comment | Organism |
---|---|---|---|
3.5.1.11 | synthesis | production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.11 | expressed in Bacillus megaterium MS941, strain is deficient in the major extracellular protease NprM due to deletion of the corresponding gene, expressed in the newly constructed Bacillus megaterium YYBm1 strain, which has a xylose utilization deficiency, 7fold higher enzyme expression compared with host strain | Priestia megaterium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.11 | additional information | leader peptide was replaced by the Bacillus megaterium LipA counterpart, changing the signal peptide increases the amount of secreted enzyme | Priestia megaterium |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.5.1.11 | extracellular | - |
Priestia megaterium | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.11 | additional information | 2.5 mM CaCl2 in the medium is optimal for enzyme production | Priestia megaterium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.11 | 27000 | - |
1 * 27000 + 1 * 57000, alpha, beta subunits produced by autoproteolytic cleavage, SDS-PAGE | Priestia megaterium |
3.5.1.11 | 27753 | - |
1 * 27753 + 1 * 61394, alpha,beta, calculated from the deduced amino acid sequence | Priestia megaterium |
3.5.1.11 | 57000 | - |
1 * 27000 + 1 * 57000, alpha, beta subunits produced by autoproteolytic cleavage, SDS-PAGE | Priestia megaterium |
3.5.1.11 | 61394 | - |
1 * 27753 + 1 * 61394, alpha,beta, calculated from the deduced amino acid sequence | Priestia megaterium |
3.5.1.11 | 90000 | - |
- |
Priestia megaterium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.11 | additional information | Priestia megaterium | autoproteolytic processing of the alpha and beta subunit | ? | - |
? | |
3.5.1.11 | penicillin G + H2O | Priestia megaterium | function in nature not fully understood, possibly functions to degrade phenylacetylated compounds in order to generate phenylacetic acid which can be used as carbon source | 6-aminopenicillanic acid + phenylacetic acid | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.11 | Priestia megaterium | - |
strain ATCC 14945, grown on buffered mineral medium containing calcium ions and defined amino acid supplements for optimal PGA production | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.5.1.11 | proteolytic modification | autoproteolytic cleavage which results in the formation of two subunits, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 with calculated molecular masses of 27753 Da and 61394 Da, respectively | Priestia megaterium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.11 | 3-nitro-6-phenylacetaminobenzoic acid + H2O | - |
Priestia megaterium | 6-nitrophenol + ? | - |
? | |
3.5.1.11 | additional information | autoproteolytic processing of the alpha and beta subunit | Priestia megaterium | ? | - |
? | |
3.5.1.11 | penicillin G + H2O | function in nature not fully understood, possibly functions to degrade phenylacetylated compounds in order to generate phenylacetic acid which can be used as carbon source | Priestia megaterium | 6-aminopenicillanic acid + phenylacetic acid | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.11 | dimer | 1 * 27000 + 1 * 57000, alpha, beta subunits produced by autoproteolytic cleavage, SDS-PAGE | Priestia megaterium |
3.5.1.11 | dimer | 1 * 27753 + 1 * 61394, alpha,beta, calculated from the deduced amino acid sequence | Priestia megaterium |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.11 | penicillin G acylase | - |
Priestia megaterium |
3.5.1.11 | PGA | - |
Priestia megaterium |