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Literature summary extracted from

  • Dutta, T.; Sengupta, R.; Sahoo, R.; Sinha Ray, S.; Bhattacharjee, A.; Ghosh, S.
    A novel cellulase free alkaliphilic xylanase from alkali tolerant Penicillium citrinum: production, purification and characterization (2007), Lett. Appl. Microbiol., 44, 206-211.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.8 2-mercaptoethanol
-
 Penicillium citrinum
3.2.1.8 dithiothreitol
-
 Penicillium citrinum
3.2.1.8 NaCl
-
 Penicillium citrinum
3.2.1.8 SDS
-
 Penicillium citrinum
3.2.1.8 Triton X 100
-
 Penicillium citrinum
3.2.1.8 Tween 20
-
 Penicillium citrinum
3.2.1.8 Urea
-
 Penicillium citrinum

Application

EC Number Application Comment Organism
3.2.1.8 industry used for pulp bleaching Penicillium citrinum
3.2.1.8 paper production used for paper bleaching Penicillium citrinum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+ slight inhibition at 3 or 6 mM Penicillium citrinum
3.2.1.8 Fe3+ slight inhibition at 3 or 6 mM Penicillium citrinum
3.2.1.8 Hg2+ strong inhibitory effect Penicillium citrinum
3.2.1.8 Mg2+ slight inhibition at 3 or 6 mM Penicillium citrinum
3.2.1.8 Mn2+ strong inhibitory effect Penicillium citrinum
3.2.1.8 additional information EDTA, Ca2+ and Ni2+ do not show any effect on xylanase activity Penicillium citrinum
3.2.1.8 Zn2+ slight inhibition at 3 or 6 mM Penicillium citrinum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 22000
-
 analytical gel filtration Penicillium citrinum
3.2.1.8 25000
-
 SDS-PAGE Penicillium citrinum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Penicillium citrinum
-
-
-
3.2.1.8 Penicillium citrinum MTCC 6489
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 Phenyl Sepharose chromatography and Superdex-200 HR Superdex-200 gel filtration Penicillium citrinum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 25.4
-
 from crude cell extract Penicillium citrinum
3.2.1.8 360.7
-
 after 14.2fold purification Penicillium citrinum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
 Penicillium citrinum xylose + ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Penicillium citrinum MTCC 6489 xylose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Penicillium citrinum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 50
-
 at both pH 3.5 and pH 8.5 Penicillium citrinum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8
-
 50 the retention of 50% of its maximum activity is observed at 0°C, retains 65% of its activity after 1-4 h incubation at 50°C Penicillium citrinum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 8.5
-
-
 Penicillium citrinum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 4 10
-
 Penicillium citrinum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Penicillium citrinum isoelectric focusing
-
 3.6