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Literature summary extracted from

  • Pan, Y.H.; Bahnson, B.J.
    Structural basis for bile salt inhibition of pancreatic phospholipase A2 (2007), J. Mol. Biol., 369, 439-450.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.4 cholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa
3.1.1.4 glycochenodeoxycholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa
3.1.1.4 glycocholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa
3.1.1.4 taurochenodeoxycholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa
3.1.1.4 taurocholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.4 pancreatic PLA2 in complex with five different naturally occurring bile salts, i.e. cholate, taurocholate, glycocholate, glycochenodeoxycholate, and taurochenodeoxycholate, in presence or absence of active-site-directed tetrahedral mimic MJ33, hanging drop vapour diffusion method, 15 mg/ml protein solution with 10 mM CaCl2, 3 mM MJ33, and 3 mM bile salt, is mixed with an equal volume of 0.001 ml of cyrstallization solution containing 0.2 M CaCl2, 0.1 M HEPES, pH 7.5, and 28% v/v PEG 400 or 0.1 M sodium cacodylate, pH 6.5, and 1.4 M sodium acetate, 1 week to 2 months, X-ray diffraction structure determination and analysis at 1.85-2.20 A resolution, modeling Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.4 cholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa
3.1.1.4 glycochenodeoxycholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa
3.1.1.4 glycocholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa
3.1.1.4 MJ33 i.e. 1-hexadecyl-3-(trifluoroethyl)-rac-glycero-2-phosphomethanol, an active-site-directed tetrahedral mimic Sus scrofa
3.1.1.4 taurochenodeoxycholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa
3.1.1.4 taurocholate bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.4 additional information Sus scrofa bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.4 Sus scrofa P00592
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.4 pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.4 additional information bile salt regulation of lipid metabolism via both activation and inhibition of PLA2, interaction and binding structure analysis, overview Sus scrofa ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.4 pancreatic phospholipase A2
-
 Sus scrofa