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Literature summary extracted from

  • Berrisford, J.M.; Hounslow, A.M.; Akerboom, J.; Hagen, W.R.; Brouns, S.J.; van der Oost, J.; Murray, I.A.; Michael Blackburn, G.; Waltho, J.P.; Rice, D.W.; Baker, P.J.
    Evidence supporting a cis-enediol-based mechanism for Pyrococcus furiosus phosphoglucose isomerase (2006), J. Mol. Biol., 358, 1353-1366.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.9 in presence of bound zinc, substrate D-fructose 6-phosphate and several competitive inhibitors Pyrococcus furiosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.9 5-phospho-D-arabinonate competitive Pyrococcus furiosus
5.3.1.9 5-phospho-D-arabinonohydroxamate competitive, stable analogue of putative cis-endiol intermediate Pyrococcus furiosus
5.3.1.9 EDTA 100fold excess, complete loss of activity within 10 min. 93% of activity may be recovered by additon of 1000fold excess of Zn2+ Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.9 0.5
-
D-fructose 6-phosphate 50°C Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.9 Pyrococcus furiosus P83194
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.9
-
Pyrococcus furiosus

Reaction

EC Number Reaction Comment Organism Reaction ID
5.3.1.9 alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate cis-endiol intermediate based mechanism with Glu97 acting as the catalytic base responsible for isomerization Pyrococcus furiosus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
5.3.1.9 enzyme inactivated by 100fold excess of EDTA can be reactivated by 1000fold excess of of Zn2+ Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.9 D-fructose 6-phosphate
-
Pyrococcus furiosus D-glucose 6-phosphate
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.9 8.4
-
D-fructose 6-phosphate 50°C Pyrococcus furiosus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.9 0.005
-
5-phospho-D-arabinonohydroxamate 50°C Pyrococcus furiosus
5.3.1.9 0.27
-
5-phospho-D-arabinonate 50°C Pyrococcus furiosus