Literature summary extracted from
Berrisford, J.M.; Hounslow, A.M.; Akerboom, J.; Hagen, W.R.; Brouns, S.J.; van der Oost, J.; Murray, I.A.; Michael Blackburn, G.; Waltho, J.P.; Rice, D.W.; Baker, P.J.
Evidence supporting a cis-enediol-based mechanism for Pyrococcus furiosus phosphoglucose isomerase (2006), J. Mol. Biol., 358, 1353-1366.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.3.1.9 |
in presence of bound zinc, substrate D-fructose 6-phosphate and several competitive inhibitors |
Pyrococcus furiosus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
5.3.1.9 |
5-phospho-D-arabinonate |
competitive |
Pyrococcus furiosus |
|
5.3.1.9 |
5-phospho-D-arabinonohydroxamate |
competitive, stable analogue of putative cis-endiol intermediate |
Pyrococcus furiosus |
|
5.3.1.9 |
EDTA |
100fold excess, complete loss of activity within 10 min. 93% of activity may be recovered by additon of 1000fold excess of Zn2+ |
Pyrococcus furiosus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
5.3.1.9 |
0.5 |
- |
D-fructose 6-phosphate |
50°C |
Pyrococcus furiosus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.3.1.9 |
Pyrococcus furiosus |
P83194 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.3.1.9 |
- |
Pyrococcus furiosus |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
5.3.1.9 |
alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate |
cis-endiol intermediate based mechanism with Glu97 acting as the catalytic base responsible for isomerization |
Pyrococcus furiosus |
|
Renatured (Commentary)
EC Number |
Renatured (Comment) |
Organism |
---|
5.3.1.9 |
enzyme inactivated by 100fold excess of EDTA can be reactivated by 1000fold excess of of Zn2+ |
Pyrococcus furiosus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.3.1.9 |
D-fructose 6-phosphate |
- |
Pyrococcus furiosus |
D-glucose 6-phosphate |
- |
? |
|
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
5.3.1.9 |
8.4 |
- |
D-fructose 6-phosphate |
50°C |
Pyrococcus furiosus |
|
Ki Value [mM]
EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Comment |
Organism |
Structure |
---|
5.3.1.9 |
0.005 |
- |
5-phospho-D-arabinonohydroxamate |
50°C |
Pyrococcus furiosus |
|
5.3.1.9 |
0.27 |
- |
5-phospho-D-arabinonate |
50°C |
Pyrococcus furiosus |
|