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Literature summary extracted from

  • Ursby, T.; Adinolfi, B.S.; Al-Karadaghi, S.; De Vendittis, E.; Bocchini, V.
    Iron superoxide dismutase from the archaeon Sulfolobus solfataricus: analysis of structure and thermostability (1999), J. Mol. Biol., 286, 189-205.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.15.1.1 purified native and recombinant enzyme, hanging drop vapour diffusion method, 21°C, 2 mg/ml protein, from 8% v/v PEG 8000, 0.1 M Tris-HCl, pH 8.5, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Fe2+ Fe-type SOD, helices alpha1 and alpha2 contribute one metal ligand each, i.e. His33 and His84, binding structure, the iron is ligated by Nepsilon2 of His33, His84 and His174, by Odelta1 of Asp170, and a solvent molecule forming a distorted trigonal bipyramidal coordination sphere, overview Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.15.1.1 24000
-
 4 * 24000, alpha2beta2, SDS-PAGE Saccharolobus solfataricus
1.15.1.1 87900
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+ Saccharolobus solfataricus superoxide dismutase is a key enzyme for the protection of aerobic organisms against toxic radicals produced during oxidative processes O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 O2.- + 2 H+ superoxide dismutase is a key enzyme for the protection of aerobic organisms against toxic radicals produced during oxidative processes Saccharolobus solfataricus O2 + H2O2
-
 ?
1.15.1.1 2 O2.- + 2 H+ dismutation of superoxide in a two-step reaction: 1. O2.- + Fe3+-SOD = O2 + Fe2+-SOD, 2. O2.- + Fe2+-SOD + 2 H+ = H2O2 + Fe3+-SOD Saccharolobus solfataricus O2 + H2O2
-
 ?
1.15.1.1 additional information unusual covalent modification of the conserved Tyr41 in the active site, interactions Tyr41-His155, overview Saccharolobus solfataricus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 tetramer 4 * 24000, alpha2beta2, SDS-PAGE Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 SOD
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 28
-
 assay at Saccharolobus solfataricus