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Literature summary extracted from

  • Singh, R.S.; Dhaliwal, R.; Puri, M.
    Partial purification and characterization of exoinulinase from Kluyveromyces marxianus YS-1 for preparation of high-fructose syrup (2007), J. Microbiol. Biotechnol., 17, 733-738.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.80 synthesis the enzyme preparation is used to hydrolyze pure inulin and raw inulin from Asparagus racemosus for the preparation of a high-fructose syrup Kluyveromyces marxianus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 Ag+ potent inhibitor below 1 mM Kluyveromyces marxianus
3.2.1.80 CuSO4 1 mM, 47% inhibition Kluyveromyces marxianus
3.2.1.80 EDTA 1 mM, 50% inhibition Kluyveromyces marxianus
3.2.1.80 Fe2(SO4)3 1 mM, 38% inhibition Kluyveromyces marxianus
3.2.1.80 Hg2+ potent inhibitor below 1mM Kluyveromyces marxianus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.80 2.7
-
 raffinose pH 5.5, 50°C Kluyveromyces marxianus
3.2.1.80 3.4
-
 inulin pH 5.5, 50°C Kluyveromyces marxianus
3.2.1.80 25.3
-
 sucrose pH 5.5, 50°C Kluyveromyces marxianus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.80 extracellular
-
 Kluyveromyces marxianus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.80 Ca2+ maximal increase in activity: 1.2fold Kluyveromyces marxianus
3.2.1.80 Mn2+ maximal increase in activity: 2.4fold Kluyveromyces marxianus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Kluyveromyces marxianus
-
-
-
3.2.1.80 Kluyveromyces marxianus Ys-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 partial Kluyveromyces marxianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.80 413
-
-
 Kluyveromyces marxianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + H2O
-
 Kluyveromyces marxianus D-glucose + D-fructose + ?
-
 ?
3.2.1.80 inulin + H2O
-
 Kluyveromyces marxianus Ys-1 D-glucose + D-fructose + ?
-
 ?
3.2.1.80 raffinose + H2O hydrolyzed at 69% of the activity with inulin Kluyveromyces marxianus beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
3.2.1.80 raffinose + H2O hydrolyzed at 69% of the activity with inulin Kluyveromyces marxianus Ys-1 beta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
-
 ?
3.2.1.80 sucrose + H2O hydrolyzed at 177.6% of the activity with inulin Kluyveromyces marxianus D-fructose + D-glucose
-
 ?
3.2.1.80 sucrose + H2O hydrolyzed at 177.6% of the activity with inulin Kluyveromyces marxianus Ys-1 D-fructose + D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 exoinulinase
-
 Kluyveromyces marxianus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 50
-
-
 Kluyveromyces marxianus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.80 50
-
 3 h, stable Kluyveromyces marxianus
3.2.1.80 60
-
 3 h, 50% loss of activity Kluyveromyces marxianus
3.2.1.80 70
-
 3 h, 85% loss of activity Kluyveromyces marxianus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 5.5
-
-
 Kluyveromyces marxianus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.80 5 6 50°C, 3 h, stable Kluyveromyces marxianus