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Literature summary extracted from

  • Huang, J.; Furusawa, T.; Sadakane, K.; Sugimura, Y.
    Purification and properties of two types of soluble trehalases from embryonic larvae of the silkworm, Bombyx mori (2006), J. Insect Biotechnol. Sericol., 75, 1-8.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 0.44
-
 trehalose soluble trehalase P II, inacetate buffer, pH 5.5, at 30°C Bombyx mori
3.2.1.28 1.56
-
 trehalose soluble trehalase P I, in acetate buffer, pH 5.5, at 30°C Bombyx mori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 73000
-
 soluble trehalase P I, SDS-PAGE Bombyx mori
3.2.1.28 140000
-
 soluble trehalase P II, SDS-PAGE Bombyx mori

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Bombyx mori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 Sephacryl S-300 gel filtration, DEAE-cellulofine column chromatography, and Con A-Sepharose column chromatography Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 egg
-
 Bombyx mori
-
3.2.1.28 embryo
-
 Bombyx mori
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 0.01
-
 soluble trehalase P I from supernatant, in acetate buffer, pH 5.5, at 30°C Bombyx mori
3.2.1.28 0.01
-
 soluble trehalase P II from supernatant, in acetate buffer, pH 5.5, at 30°C Bombyx mori
3.2.1.28 1.03
-
 soluble trehalase P I after 86.9fold purification, in acetate buffer, pH 5.5, at 30°C Bombyx mori
3.2.1.28 2.9
-
 soluble trehalase P II after 245.7fold purification, in acetate buffer, pH 5.5, at 30°C Bombyx mori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 trehalose + H2O
-
 Bombyx mori D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 acid trehalase
-
 Bombyx mori
3.2.1.28 alpha,alpha glucoside 1-glucohydrolase
-
 Bombyx mori
3.2.1.28 soluble P I type trehalase
-
 Bombyx mori
3.2.1.28 soluble P II type trehalase
-
 Bombyx mori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 40 45 soluble trehalase P I Bombyx mori
3.2.1.28 50
-
 above 50°C, soluble trehalase P I Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 5.5
-
 soluble trehalase P II Bombyx mori
3.2.1.28 6.5
-
 soluble trehalase P I Bombyx mori