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Literature summary extracted from

  • Gulati, H.K.; Chadha, B.S.; Saini, H.S.
    Production and characterization of thermostable alkaline phytase from Bacillus laevolacticus isolated from rhizosphere soil (2007), J. Ind. Microbiol. Biotechnol., 34, 91-98.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.72 additional information sucrose poorly supports phytase production. Maximal phytase yield is obtained with 2% (v/v) inoculum, phytase activity decreases at lower and higher inoculum concentrations Sporolactobacillus laevolacticus
3.1.3.72 NH4+ H2PO4- can stimulate high-phytase production Sporolactobacillus laevolacticus

Application

EC Number Application Comment Organism
3.1.3.72 agriculture partially purified enzyme shows properties that are suitable for animal feed applications Sporolactobacillus laevolacticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.72 CuSO4 moderately inhibits phytase activity Sporolactobacillus laevolacticus
3.1.3.72 EDTA at 1 mM inhibits the enzyme activity up to 90% and completely at 5 mM concentration Sporolactobacillus laevolacticus
3.1.3.72 additional information MnSO4, CaCl2, MgSO4, NaCl, KCl, BaCl2 and CoCl2 show insignificant effect on enzyme activity Sporolactobacillus laevolacticus
3.1.3.72 ZnSO4 moderately inhibits phytase activity Sporolactobacillus laevolacticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.72 0.526
-
 Na-phytate
-
 Sporolactobacillus laevolacticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.72 Ca2+ Ca2+ dependent Sporolactobacillus laevolacticus
3.1.3.72 Na+
-
 Sporolactobacillus laevolacticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.72 41000
-
 1 * 41000, SDS-PAGE, 1 * 46000, SDS-PAGE Sporolactobacillus laevolacticus
3.1.3.72 46000
-
 1 * 41000, SDS-PAGE, 1 * 46000, SDS-PAGE Sporolactobacillus laevolacticus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.72 Sporolactobacillus laevolacticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.72 partially purified, 6.5fold, by gel filtration Sporolactobacillus laevolacticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.72 1.94
-
 crude extract Sporolactobacillus laevolacticus
3.1.3.72 12.69
-
 partially purified phytase Sporolactobacillus laevolacticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.72 ADP + H2O 103% relative activity Sporolactobacillus laevolacticus ?
-
 ?
3.1.3.72 AMP + H2O 5% relative activity Sporolactobacillus laevolacticus ?
-
 ?
3.1.3.72 ATP + H2O 41% relative activity Sporolactobacillus laevolacticus ?
-
 ?
3.1.3.72 Ca-phytate + H2O 133% relative activity Sporolactobacillus laevolacticus ?
-
 ?
3.1.3.72 additional information no activity with riboflurin phosphate Sporolactobacillus laevolacticus ?
-
 ?
3.1.3.72 myo-inositol-1,2,3,4,5,6-hexakisphosphate + H2O
-
 Sporolactobacillus laevolacticus myo-inositol-1,2,3,4,6-pentakisphosphate + phosphate
-
 ?
3.1.3.72 Na-phytate + H2O 100% relative activity Sporolactobacillus laevolacticus ?
-
 ?
3.1.3.72 p-nitrophenyl phosphate + H2O 5% relative activity Sporolactobacillus laevolacticus p-nitrophenol + phosphate
-
 ?
3.1.3.72 phenyl phosphate + H2O 32% relative activity Sporolactobacillus laevolacticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.72 ? 1 * 41000, SDS-PAGE, 1 * 46000, SDS-PAGE Sporolactobacillus laevolacticus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.72 alkaline phytase
-
 Sporolactobacillus laevolacticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.72 70
-
 partially purified phytase Sporolactobacillus laevolacticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.72 60
-
 ca. 80% activity at pH 8.0 for up to 3 h in the presence/absence of 5 mM CaCl2. In the presence/absence of 5 mM CaCl2, phytase is also stable at 80°C and shows ca. 100% of residual activity for 30 min at pH 8.0 and then declines sharply thereafter Sporolactobacillus laevolacticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.72 7
-
 partially purified phytase Sporolactobacillus laevolacticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.72 3.5 8.5 is catalytically active under alkaline pH range 8-10, showing 90% of the optimal activity Sporolactobacillus laevolacticus