EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.21 | expression of wild-type and mutant hybrid of BGL1-CBD2 domain with cellobiohydrolase CBHII in in Saccharomyces cerevisiae strain CENPK42 under the control of phosphoglycerate-kinase-1 promoter PGK1P and terminator PGK1T and yeast mating pheromone alpha-factor secretion signal MFalpha1S, subcloning in Escherichia coli | Saccharomycopsis fibuligera |
3.2.1.91 | the cellulose-binding domain 2 of CBHII is fused to the beta-glucosidase enzyme from Saccharomycopsis fibuligera and expressed in Saccharomyces cerevisiae strain CENPK42 | Trichoderma reesei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.21 | additional information | fusion of BGL1 to the cellulose-binding domain CBD2 of Trichoderma reesei cellobiohydrolase CBHII, recombinant enzymes are constructed with single and double copies of CBD2 fused at the N-terminus of BGL1 to mimic the two-domain organization displayed by cellulolytic enzymes in nature, the mutant shows a 2-4fold increased activity compared to the wild-type enzyme | Saccharomycopsis fibuligera |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | additional information | - |
additional information | kinetics of wild-type and mutant enzymes | Saccharomycopsis fibuligera |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 220000 | - |
recombinant wild-type enzyme | Saccharomycopsis fibuligera |
3.2.1.21 | 225000 | 230000 | recombinant mutant enzyme | Saccharomycopsis fibuligera |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Saccharomycopsis fibuligera | - |
- |
- |
3.2.1.91 | Trichoderma reesei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.91 | DEAE Sepharose column chromatography | Trichoderma reesei |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | additional information | - |
- |
Saccharomycopsis fibuligera |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Saccharomycopsis fibuligera | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | avicel + H2O | - |
Saccharomycopsis fibuligera | ? | - |
? | |
3.2.1.21 | cellobiose + H2O | - |
Saccharomycopsis fibuligera | 2 beta-D-glucose | - |
? | |
3.2.1.21 | cellopentaose + H2O | - |
Saccharomycopsis fibuligera | 5 D-glucose | - |
? | |
3.2.1.21 | cellotriose + H2O | - |
Saccharomycopsis fibuligera | 3 D-glucose | - |
? | |
3.2.1.21 | additional information | BGL1 is a non-cellulolytic yeast enzyme, substrate specifiicty with beta-1,4 linkage-specific cellulosic substrates, overview | Saccharomycopsis fibuligera | ? | - |
? | |
3.2.1.91 | avicel + H2O | - |
Trichoderma reesei | cellobiose + ? | - |
? | |
3.2.1.91 | carboxymethylcellulose + H2O | - |
Trichoderma reesei | cellobiose + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.21 | BGL1 | - |
Saccharomycopsis fibuligera |
3.2.1.91 | CBHII | - |
Trichoderma reesei |
3.2.1.91 | cellobiohydrolase | - |
Trichoderma reesei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 22 | - |
assay at room temperature, substrate cellobiose | Saccharomycopsis fibuligera |
3.2.1.21 | 37 | - |
assay at, substrate 4-nitrophenyl-beta-D-glucopyranoside | Saccharomycopsis fibuligera |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | additional information | - |
temperature profile | Saccharomycopsis fibuligera |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 4.5 | - |
assay at, substrate cellobiose | Saccharomycopsis fibuligera |
3.2.1.21 | 5 | - |
assay at, substrate 4-nitrophenyl-beta-D-glucopyranoside | Saccharomycopsis fibuligera |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | additional information | - |
pH profile | Saccharomycopsis fibuligera |