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Literature summary extracted from

  • Mennink-Kersten, M.A.; Ruegebrink, D.; Wasei, N.; Melchers, W.J.; Verweij, P.E.
    In vitro release by Aspergillus fumigatus of galactofuranose antigens, 1,3-beta-D-glucan, and DNA, surrogate markers used for diagnosis of invasive aspergillosis (2006), J. Clin. Microbiol., 44, 1711-1718.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.157 overexpressed in Escherichia coli BL21(DE3) Alteromonas fortis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.146 Aspergillus fumigatus
-
-
-
3.2.1.157 Alteromonas fortis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.157 purified by affinity chromoatography Alteromonas fortis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.146 additional information
-
 enzyme activity is low during logarithmic growth of a culture medium of Aspergillus fumigatus (about 0.2 to 0.4 U/liter) and increases during the lytical phase. Maximal activity is the lowest at higher pHs, being 1.4 U/liter at pH 7.1 to 7.4 and 2.6 U/liter at pH 7.4 and higher at low pHs, being 5.4 U/liter at pH 3.6 to 4.4 and 4.9 U/liter at pH 5.0. Activity is even higher when grown on 25 mM of glucose at pH 5.0, being 11.6 U/liter Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.146 p-nitrophenyl-beta-D-galactofuranoside + H2O
-
 Aspergillus fumigatus p-nitrophenol + beta-D-galactofuranose
-
 ?
3.2.1.157 iota-carrageenan + H2O hybrid kappa/iota-carrageeans extracted from Gigartina skottsbergii, Chondracanthus chamissoi, and Chondrus crispus are incubated with Pseudoalteromonas carrageenovora kappa-carrageenase and Alteromonas fortis iota-carrageenase. A low percentage of degradation is observed after treatment by iota-carrageenase, suggesting that long segments of iota-carrabiose or block of iota-carrageenan are low in abundance Alteromonas fortis hydrolyzed iota-carrageenan
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.146 beta-galactofuranosidase
-
 Aspergillus fumigatus
3.2.1.157 iota-carrageenase
-
 Alteromonas fortis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.146 37
-
-
 Aspergillus fumigatus
3.2.1.157 40
-
-
 Alteromonas fortis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.146 4
-
-
 Aspergillus fumigatus
3.2.1.157 7.5
-
 assay at Alteromonas fortis