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Literature summary extracted from

  • Paula, A.V.; Urioste, D.; Santos, J.C.; de Castro, H.F.
    Porcine pancreatic lipase immobilized on polysiloxane-polyvinyl alcohol hybrid matrix: catalytic properties and feasibility to mediate synthesis of surfactants and biodiesel (2007), J. Chem. Technol. Biotechnol., 82, 281-288.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.3 synthesis application of the immobilized lipase in non-conventional biocatalysis for the synthesis of surfactants and biodiesel, overview Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 additional information immobilization of the pancreatic lipase on a polysiloxane-polyvinyl alcohol hybrid matrix with 250 U/g matrix, the immobilized enzyme shows high activity converting triglycerides to fatty acid alkyl esters attaining yields varying from 75% to 95% Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 330
-
 olive oil pH 7.0, 37°C, immobilized lipase Sus scrofa
3.1.1.3 534
-
 olive oil pH 7.0, 37°C, free lipase Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Sus scrofa P00591
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 commercial preparation from pancreas Sus scrofa
-
3.1.1.3 pancreas commercial preparation Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 additional information
-
-
 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 olive oil + H2O
-
 Sus scrofa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 Pancreatic lipase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
 free lipase Sus scrofa
3.1.1.3 45
-
 immobilized lipase Sus scrofa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 40 60 activity range of free lipase and immobilized lipase, over 80% of maximal activity at 35°C-55°C of the immobilized lipase Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 45
-
 1 h, the free lipase shows 20% remaining activity Sus scrofa
3.1.1.3 50
-
 1 h, complete inactivation of the free lipase Sus scrofa
3.1.1.3 60
-
 1 h, the immobilized lipase shows 14.3% remaining activity Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8
-
 free lipase and immobilized lipase Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 additional information
-
 the immobilization procedure broadened the pH working range of the free lipase Sus scrofa
3.1.1.3 7 8.5 over 80% of maximal activity within this range, immobilized lipase Sus scrofa