EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.6.8 | medicine | availability of AS-A on the sperm surface is important for the dispersion of cumulus layers of cumulus oocyte complexes | Mus musculus |
3.1.6.8 | medicine | availability of AS-A on the sperm surface is important for the dispersion of cumulus layers of cumulus oocyte complexes | Sus scrofa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.6.1 | additional information | sperm of enzyme deficient mice shows a significant delay in dispoersion of expanded cumulus oocyte complexes. Within 1 h of sperm-cumulus oocyte complex co-incubation, the size of cumulus oocyte complexes incubated with enzyme deficient sperm is 65% of its original dimension, whereas that of cumulus oocyte complexes inseminated with wild-type sperm is only 17% | Mus musculus |
3.1.6.8 | additional information | sperm from AS-A null mice show a significant delay in cumulus oocyte complex dispersion, compared with wild-type sperm | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.6.8 | anti-AS-A IgG | dispersion of cumulus layers of cumulus oocyte complexes does not occurr in co-incubates with anti-AS-A IgG treated sperm and in the presence of apigenin | Mus musculus | |
3.1.6.8 | additional information | dispersion of cumulus layers of cumulus oocyte complexes still occur in co-incubates with preimmune IgG treated sperm | Mus musculus | |
3.1.6.8 | additional information | cumulus oocyte complex dispersion caused by AS-A is the same in the presence or absence of apigenin | Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.6.8 | 66000 | - |
1 * 66000, SDS-PAGE | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.6.1 | additional information | Mus musculus | enzyme is able to disperse the cumulus matrix of expanded cumulus oocyte complexes, this dispersion action is not associated with desulfation activity | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.6.1 | Mus musculus | - |
- |
- |
3.1.6.8 | Mus musculus | - |
- |
- |
3.1.6.8 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.6.8 | by gel filtration | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.6.1 | sperm | localized at sperm head surface. Enzyme is able to disperse the cumulus matrix of expanded cumulus oocyte complexes, this dispersion action is not associated with desulfation activity | Mus musculus | - |
3.1.6.8 | sperm | - |
Mus musculus | - |
3.1.6.8 | sperm | - |
Sus scrofa | - |
3.1.6.8 | testis | - |
Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.6.8 | 5 | - |
degraded enzyme | Sus scrofa |
3.1.6.8 | 24 | - |
66 kDa form | Sus scrofa |
3.1.6.8 | 60 | - |
purified enzyme, 66 kDa form | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.6.1 | additional information | enzyme is able to disperse the cumulus matrix of expanded cumulus oocyte complexes, this dispersion action is not associated with desulfation activity | Mus musculus | ? | - |
? | |
3.1.6.8 | chondroitin sulfate B + H2O | - |
Sus scrofa | sulfate | - |
? | |
3.1.6.8 | hyaluronan + H2O | - |
Sus scrofa | ? | - |
? | |
3.1.6.8 | additional information | is able to disperse cumulus layers of cumulus oocyte complexes. Chondroitin sulfate A, chondroitin sulfate C, ovalbumin and heparan sulfate do not show interaction with AS-A | Sus scrofa | ? | - |
? | |
3.1.6.8 | p-nitrocatechol sulfate + H2O | - |
Sus scrofa | p-nitrocatechol + sulfate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.6.1 | More | enzyme has a specific affinity for chondroitin sulfate B, a component of the cumulus matrix proteoglycan network | Mus musculus |
3.1.6.8 | monomer | 1 * 66000, SDS-PAGE | Sus scrofa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.6.8 | arylsulfatase A | - |
Mus musculus |
3.1.6.8 | arylsulfatase A | - |
Sus scrofa |
3.1.6.8 | AS-A | - |
Mus musculus |
3.1.6.8 | AS-A | - |
Sus scrofa |