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Literature summary extracted from

  • Wu, A.; Anupriwan, A.; Iamsaard, S.; Chakrabandhu, K.; Santos, D.C.; Rupar, T.; Tsang, B.K.; Carmona, E.; Tanphaichitr, N.
    Sperm surface arylsulfatase A can disperse the cumulus matrix of cumulus oocyte complexes (2007), J. Cell. Physiol., 213, 201-211.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.6.8 medicine availability of AS-A on the sperm surface is important for the dispersion of cumulus layers of cumulus oocyte complexes Mus musculus
3.1.6.8 medicine availability of AS-A on the sperm surface is important for the dispersion of cumulus layers of cumulus oocyte complexes Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.6.1 additional information sperm of enzyme deficient mice shows a significant delay in dispoersion of expanded cumulus oocyte complexes. Within 1 h of sperm-cumulus oocyte complex co-incubation, the size of cumulus oocyte complexes incubated with enzyme deficient sperm is 65% of its original dimension, whereas that of cumulus oocyte complexes inseminated with wild-type sperm is only 17% Mus musculus
3.1.6.8 additional information sperm from AS-A null mice show a significant delay in cumulus oocyte complex dispersion, compared with wild-type sperm Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.8 anti-AS-A IgG dispersion of cumulus layers of cumulus oocyte complexes does not occurr in co-incubates with anti-AS-A IgG treated sperm and in the presence of apigenin Mus musculus
3.1.6.8 additional information dispersion of cumulus layers of cumulus oocyte complexes still occur in co-incubates with preimmune IgG treated sperm Mus musculus
3.1.6.8 additional information cumulus oocyte complex dispersion caused by AS-A is the same in the presence or absence of apigenin Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.8 66000
-
1 * 66000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.1 additional information Mus musculus enzyme is able to disperse the cumulus matrix of expanded cumulus oocyte complexes, this dispersion action is not associated with desulfation activity ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.1 Mus musculus
-
-
-
3.1.6.8 Mus musculus
-
-
-
3.1.6.8 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.8 by gel filtration Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.1 sperm localized at sperm head surface. Enzyme is able to disperse the cumulus matrix of expanded cumulus oocyte complexes, this dispersion action is not associated with desulfation activity Mus musculus
-
3.1.6.8 sperm
-
Mus musculus
-
3.1.6.8 sperm
-
Sus scrofa
-
3.1.6.8 testis
-
Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.6.8 5
-
degraded enzyme Sus scrofa
3.1.6.8 24
-
66 kDa form Sus scrofa
3.1.6.8 60
-
purified enzyme, 66 kDa form Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.1 additional information enzyme is able to disperse the cumulus matrix of expanded cumulus oocyte complexes, this dispersion action is not associated with desulfation activity Mus musculus ?
-
?
3.1.6.8 chondroitin sulfate B + H2O
-
Sus scrofa sulfate
-
?
3.1.6.8 hyaluronan + H2O
-
Sus scrofa ?
-
?
3.1.6.8 additional information is able to disperse cumulus layers of cumulus oocyte complexes. Chondroitin sulfate A, chondroitin sulfate C, ovalbumin and heparan sulfate do not show interaction with AS-A Sus scrofa ?
-
?
3.1.6.8 p-nitrocatechol sulfate + H2O
-
Sus scrofa p-nitrocatechol + sulfate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.6.1 More enzyme has a specific affinity for chondroitin sulfate B, a component of the cumulus matrix proteoglycan network Mus musculus
3.1.6.8 monomer 1 * 66000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
3.1.6.8 arylsulfatase A
-
Mus musculus
3.1.6.8 arylsulfatase A
-
Sus scrofa
3.1.6.8 AS-A
-
Mus musculus
3.1.6.8 AS-A
-
Sus scrofa