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Literature summary extracted from

  • Boyce, A.; Walsh, G.
    Purification and characterisation of an acid phosphatase with phytase activity from Mucor hiemalis Wehmer (2007), J. Biotechnol., 132, 82-87.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.434
-
 p-nitrophenyl phosphate estimated Km Mucor hiemalis
3.1.3.2 1.29
-
 sodium phytate estimated Km Mucor hiemalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 30000
-
 native enzyme, gel filtration Mucor hiemalis
3.1.3.2 45000
-
 purified enzyme, SDS-PAGE Mucor hiemalis

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Mucor hiemalis
-
 has phytase activity
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.2 glycoprotein
-
 Mucor hiemalis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 DEAE Sepharose CL-6B column chromatography, Sephacryl S-100-HR gel filtration, phenyl agarose column chromatography, and Sephadex G-25 gel filtration Mucor hiemalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 3.3
-
 crude cell extract Mucor hiemalis
3.1.3.2 46.7
-
 after 14.1fold purification Mucor hiemalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 1-naphthyl phosphate + H2O
-
 Mucor hiemalis 1-naphthol + phosphate
-
 ?
3.1.3.2 2-naphthyl phosphate + H2O
-
 Mucor hiemalis 2-naphthol + phosphate
-
 ?
3.1.3.2 ADP + H2O
-
 Mucor hiemalis AMP + phosphate
-
 ?
3.1.3.2 AMP + H2O
-
 Mucor hiemalis adenosine + phosphate
-
 ?
3.1.3.2 ATP + H2O
-
 Mucor hiemalis ADP + phosphate
-
 ?
3.1.3.2 calcium phytate + H2O
-
 Mucor hiemalis ?
-
 ?
3.1.3.2 D-glucose 1-phosphate + H2O
-
 Mucor hiemalis D-glucose + phosphate
-
 ?
3.1.3.2 glycerol 2-phosphate + H2O
-
 Mucor hiemalis glycerol + phosphate
-
 ?
3.1.3.2 p-nitrophenyl phosphate + H2O
-
 Mucor hiemalis p-nitrophenol + phosphate
-
 ?
3.1.3.2 phenyl phosphate + H2O
-
 Mucor hiemalis phenol + phosphate
-
 ?
3.1.3.2 sodium phytate + H2O
-
 Mucor hiemalis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer
-
 Mucor hiemalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 55
-
-
 Mucor hiemalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 80
-
 retains 64%, 63% and 61% of its original activity after heating for 1, 3 and 5 min, respectively, at 80°C Mucor hiemalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5 5.5
-
 Mucor hiemalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.2 6 7 retains almost 50% of optimal activity at pH 6.0, drops to 13% residual activity at pH 7.0 Mucor hiemalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.2 Mucor hiemalis isoelectric focusing
-
 7.8