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Literature summary extracted from

  • Mohamed, S.A.; Farid, N.M.; Hossiny, E.N.; Bassuiny, R.I.
    Biochemical characterization of an extracellular polygalacturonase from Trichoderma harzianum (2006), J. Biotechnol., 127, 54-64.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.15 food industry properties of the enzyme may be highly beneficial during fruit processing Trichoderma harzianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Ag+ 56.6% residual activity with 1mM Trichoderma harzianum
3.2.1.15 Ba2+ 96.3% residual activity with 1mM Trichoderma harzianum
3.2.1.15 Ca2+ 66.7% residual activity with 1mM Trichoderma harzianum
3.2.1.15 Citric acid 64% inhibition at 5 mM Trichoderma harzianum
3.2.1.15 Co2+ 1mM completely inhibits Trichoderma harzianum
3.2.1.15 Cu2+ 83.6% residual activity with 1mM Trichoderma harzianum
3.2.1.15 Fe3+ 93.2% residual activity with 1mM Trichoderma harzianum
3.2.1.15 Hg2+ 46.3% residual activity with 1mM Trichoderma harzianum
3.2.1.15 iodoacetic acid 74% inhibition at 5 mM Trichoderma harzianum
3.2.1.15 Li+ 83.6% residual activity with 1mM Trichoderma harzianum
3.2.1.15 Mg2+ 76.4% residual activity with 1mM Trichoderma harzianum
3.2.1.15 Mn2+ 1mM completely inhibits Trichoderma harzianum
3.2.1.15 additional information 1,10-phenanthroline, Tween 20, Tween 80, Triton X-100 and SDS have no effect on enzyme activity Trichoderma harzianum
3.2.1.15 Ni2+ 89.9% residual activity with 1mM Trichoderma harzianum
3.2.1.15 PMSF 78% inhibition at 5 mM Trichoderma harzianum
3.2.1.15 Zn2+ 81.7% residual activity with 1mM Trichoderma harzianum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 31000
-
 gel filtration Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Trichoderma harzianum
-
-
-
3.2.1.15 Trichoderma harzianum CECT 2413
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 13fold, to homogeneity by two chromatography steps Trichoderma harzianum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 21
-
 culture filtrate Trichoderma harzianum
3.2.1.15 276
-
 13fold purified enzyme Trichoderma harzianum

Storage Stability

EC Number Storage Stability Organism
3.2.1.15 0-4°C, stable for 3 months Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 additional information very low activity toward non-pectic polysaccharides Trichoderma harzianum ?
-
 ?
3.2.1.15 additional information very low activity toward non-pectic polysaccharides Trichoderma harzianum CECT 2413 ?
-
 ?
3.2.1.15 pectin + H2O citrus pectin, apple pectin and amylopectin, hydrolysis of different pectins is decreased with increasing of degree of esterification Trichoderma harzianum ?
-
 ?
3.2.1.15 pectin + H2O citrus pectin, apple pectin and amylopectin, hydrolysis of different pectins is decreased with increasing of degree of esterification Trichoderma harzianum CECT 2413 ?
-
 ?
3.2.1.15 polygalacturonic acid + H2O
-
 Trichoderma harzianum ?
-
 ?
3.2.1.15 polygalacturonic acid + H2O
-
 Trichoderma harzianum CECT 2413 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 monomer 1 * 31000, SDS-PAGE Trichoderma harzianum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 endoPG
-
 Trichoderma harzianum
3.2.1.15 PGII
-
 Trichoderma harzianum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 40
-
-
 Trichoderma harzianum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 30
-
 approximately stable up to 30°C for 60 min of incubation. Activity of enzyme is gradually decreased with increasing temperature and time Trichoderma harzianum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.15 592
-
 polygalacturonic acid
-
 Trichoderma harzianum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 5
-
-
 Trichoderma harzianum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 3.5 8
-
 Trichoderma harzianum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 5
-
 very stable at pH 5.0, retains 49% and 75% of its activity at pH 4.0 and 6.0, respectively. Half life time of enzymatic activity is 24 h at pH 4.0. The enzyme loses about 70-80% of its activity at pH 3.0 and pH’s from 7.0 to 11 Trichoderma harzianum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.15 Trichoderma harzianum isoelectric focusing
-
 4.5