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Literature summary extracted from

  • Voget, S.; Steele, H.L.; Streit, W.R.
    Characterization of a metagenome-derived halotolerant cellulase (2006), J. Biotechnol., 126, 26-36.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 Dimethylsulfoxide above 5-15% v/v unidentified microorganism

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 overexpressed in Escherichia coli unidentified microorganism

General Stability

EC Number General Stability Organism
3.2.1.4 high salt stability, the enzyme retained 86-87% activity after incubation with 3 M NaCl, 3 M RbCl or 4 M KCl for 20 h unidentified microorganism

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 acetonitrile 10% v/v, 89% loss of activity unidentified microorganism
3.2.1.4 Ba2+ 1 mM, 79%inhibition unidentified microorganism
3.2.1.4 Ca2+ 1 mM, 74%inhibition unidentified microorganism
3.2.1.4 Cu2+ 1 mM, 12% loss of activity unidentified microorganism
3.2.1.4 Dimethylsulfoxide 30% v/v, 50% inhibition unidentified microorganism
3.2.1.4 EDTA 50 mM, 14% inhibition unidentified microorganism
3.2.1.4 ethanol 30% v/v, 92% loss of activity unidentified microorganism
3.2.1.4 Fe3+ 1 mM, 73% inhibition unidentified microorganism
3.2.1.4 methanol 30% v/v, 62% loss of activity unidentified microorganism
3.2.1.4 Mn2+ 5 mM, 20% inhibition unidentified microorganism
3.2.1.4 SDS 1% v/v, almost complete loss of activity unidentified microorganism
3.2.1.4 Zn2+ 1 mM, 78%inhibition unidentified microorganism

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Mn2+ 1 mM, 1.3fold activation unidentified microorganism

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 42000
-
 x * 42000, SDS-PAGE unidentified microorganism
3.2.1.4 42100
-
 x * 42100, calculated from sequence unidentified microorganism

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 unidentified microorganism
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 unidentified microorganism

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 cellopentaose + H2O
-
 unidentified microorganism cellobiose + glucose
-
 ?
3.2.1.4 additional information no activity against crystalline forms of cellulose such as filter-paper or avicel and the beta-1,3-linked glucan laminarin. No activity with xylan unidentified microorganism ?
-
 ?
3.2.1.4 soluble cellulose + H2O endo mode of action. At the beginning only high molecular mass products are released suggesting an endowise action of the enzyme. The major product has a degree of polymerization of three, minor quantities of smaller and larger oligosaccharides are also produced. Not active against insoluble cellulose unidentified microorganism ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 42000, SDS-PAGE unidentified microorganism
3.2.1.4 ? x * 42100, calculated from sequence unidentified microorganism

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel5A
-
 unidentified microorganism

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 45
-
-
 unidentified microorganism

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 30 50 30°C: 76% of maximal activity, 80°C: 89% of maximal activity unidentified microorganism

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 40
-
 stable up to 11 days unidentified microorganism
3.2.1.4 55
-
 10 min, 80% loss of activity unidentified microorganism

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.5
-
-
 unidentified microorganism

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 5.5 9 pH 5.5: 60% of maximal activity, pH 9: 68% of maximal activity unidentified microorganism