Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nohara, D.; Hizikata, H.; Asami, O.
    Enhancement of the activity of renatured lysozyme by protein disulfide isomerase (2007), J. Biosci. Bioeng., 104, 235-237.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.17 Protein disulfide isomerase enhances activity of the activity of renatured lysozyme Gallus gallus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.4.1 overexpression in Bacillus brevis, secretion to the cell medium Humicola insolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.4.1 additional information
-
additional information refolding reaction obeys almost first-order kinetics with respect to PDI concentration Humicola insolens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.17 Gallus gallus
-
-
-
5.3.4.1 Humicola insolens
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.2.1.17 the refolding rate increases with an increase in concentration of protein disulfide isomerase. About 100% activity is recovered after 10 min initiation of the reaction at 0.062 mM protein disulfide isomerase, whereas several hours are needed for 100% activity recovery in absence of protein disulfide isomerase Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.17 egg white
-
Gallus gallus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.4.1 denatured lysozyme PDI catalyzes the formation, rearrangement, and breakage of disulfide bonds, oxidative refolding by PDI almost completely restores lysozyme activity, overview Humicola insolens ?
-
?
5.3.4.1 additional information PDI also functions as a dehydroascorbate reductase and a molecular chaperone besides its disulfide-isomerizing function Humicola insolens ?
-
?

Synonyms

EC Number Synonyms Comment Organism
5.3.4.1 PDI
-
Humicola insolens
5.3.4.1 protein disulfide isomerase
-
Humicola insolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.4.1 22
-
assay at room temprature Humicola insolens

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.4.1 GSH
-
Humicola insolens
5.3.4.1 GSSG
-
Humicola insolens