EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.4.2 | expression in Escherichia coli | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.4.2 | 0.0415 | - |
chorismate | pH 7.0, 30°C | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
5.4.4.2 | plastid | stromal protein | Arabidopsis thaliana | 9536 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.4.4.2 | Mg2+ | absolutely required | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.4.4.2 | 59000 | - |
gel filtration | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.4.2 | Arabidopsis thaliana | Q9S7H8 | activity is not regulated by light-dependent changes in stromal pH value, Mg2+ or redox potential | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
5.4.4.2 | proteolytic modification | precursor protein of 62000 Da is cleaved to 58000 Da upon import into plastid | Arabidopsis thaliana |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.4.4.2 | 0.241 | - |
pH 7.0, 30°C, recombinant protein | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.4.2 | Chorismate | - |
Arabidopsis thaliana | Isochorismate | reaction operates near equilibrium, equilibrium constant is 0.89. Enzyme does not convert chorismate directly to salicylic acid | r | |
5.4.4.2 | isochorismate | - |
Arabidopsis thaliana | chorismate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.4.4.2 | monomer | 1 * 59000, SDS-PAGE | Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.4.2 | 23 | - |
- |
Arabidopsis thaliana |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.4.2 | 4 | 37 | more than 90% of maximum activity within this range | Arabidopsis thaliana |
5.4.4.2 | 4 | 44 | more than 75% of maximum activity within this range | Arabidopsis thaliana |
5.4.4.2 | 70 | - |
no activity | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.4.2 | 0.645 | - |
chorismate | pH 7.0, 30°C | Arabidopsis thaliana | |
5.4.4.2 | 6.08 | - |
chorismate | pH 7.0, 30°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.4.2 | 7.5 | 8 | - |
Arabidopsis thaliana |