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Literature summary extracted from

  • Strawn, M.A.; Marr, S.K.; Inoue, K.; Inada, N.; Zubieta, C.; Wildermuth, M.C.
    Arabidopsis isochorismate synthase functional in pathogen-induced salicylate biosynthesis exhibits properties consistent with a role in diverse stress responses (2007), J. Biol. Chem., 282, 5919-5933.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.4.2 expression in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.4.2 0.0415
-
chorismate pH 7.0, 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.4.4.2 plastid stromal protein Arabidopsis thaliana 9536
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.4.2 Mg2+ absolutely required Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.4.2 59000
-
gel filtration Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
5.4.4.2 Arabidopsis thaliana Q9S7H8 activity is not regulated by light-dependent changes in stromal pH value, Mg2+ or redox potential
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
5.4.4.2 proteolytic modification precursor protein of 62000 Da is cleaved to 58000 Da upon import into plastid Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.4.2 0.241
-
pH 7.0, 30°C, recombinant protein Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.4.2 Chorismate
-
Arabidopsis thaliana Isochorismate reaction operates near equilibrium, equilibrium constant is 0.89. Enzyme does not convert chorismate directly to salicylic acid r
5.4.4.2 isochorismate
-
Arabidopsis thaliana chorismate
-
r

Subunits

EC Number Subunits Comment Organism
5.4.4.2 monomer 1 * 59000, SDS-PAGE Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.4.2 23
-
-
Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.4.2 4 37 more than 90% of maximum activity within this range Arabidopsis thaliana
5.4.4.2 4 44 more than 75% of maximum activity within this range Arabidopsis thaliana
5.4.4.2 70
-
no activity Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.4.2 0.645
-
chorismate pH 7.0, 30°C Arabidopsis thaliana
5.4.4.2 6.08
-
chorismate pH 7.0, 30°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.4.2 7.5 8
-
Arabidopsis thaliana