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Literature summary extracted from

  • Wu, Y.; Termine, D.J.; Swulius, M.T.; Moremen, K.W.; Sifers, R.N.
    Human endoplasmic reticulum mannosidase I is subject to regulated proteolysis (2007), J. Biol. Chem., 282, 4841-4849.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.209 additional information genetic-engineered removal of the amino-terminal cytoplasmic tail. Removal of the tail does not influence the remaining structure of the molecule, including the original transmembrane domain, noncleavable ER sorting signal, and proposed stem and catalytic domains. Synthesized with slightly different molecular weights, its absence did not diminish the efficiency of protein translation. Investigation of involvement of the tail by fluorescent fusion proteins Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.209 brefeldin A known inhibitor of vesicle transport, ablated intracellular turnover of the radiolabeled molecules. Plus digestion with potato acid phosphatase implies that phosphorylation of ER mannosidase I is responsible for the altered electrophoretic mobility Homo sapiens
3.2.1.209 Chloroquine lysosomotrophic amine capable of raising the pH of acidic intracellular compartments, added to the medium with weak inhibition Homo sapiens
3.2.1.209 Chloroquine lysosomotrophic amine capable of raising the pH of acidic intracellular compartments, effect on the intracellular fate of endogenous mouse ER mannosidase I Mus musculus
3.2.1.209 cycloheximide inhibitory effect like kifunensine Mus musculus
3.2.1.209 kifunensine general membrane-permeable inhibitor of alpha-mannosidase activity Homo sapiens
3.2.1.209 kifunensine general membrane-permeable inhibitor of alpha-mannosidase activity, effect on the intracellular fate of endogenous mouse ER mannosidase I Mus musculus
3.2.1.209 lactacystin irreversible inhibitor of multicatalytic proteasomes, only a minor effect on the disappearance of radiolabeled human ER mannosidase I Homo sapiens
3.2.1.209 lactacystin irreversible inhibitor of multicatalytic proteasomes, effect on the intracellular fate of endogenous mouse ER mannosidase I Mus musculus
3.2.1.209 leupeptin lysosomal protease inhibitor, intracellular turnover of pulse-radiolabeled recombinant human ER mannosidase I is substantially inhibited Homo sapiens
3.2.1.209 leupeptin lysosomal protease inhibitor, effect on the intracellular fate of endogenous mouse ER mannosidase I Mus musculus
3.2.1.209 additional information staurosporine and genistein have an effect, tesing arrested intracellular turnover in response to selective inhibition of serine kinase activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.209 endoplasmic reticulum
-
 Mus musculus 5783
-
3.2.1.209 endoplasmic reticulum
-
 Homo sapiens 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.209 79000
-
 Western blotting of cell lysates, transiently expressed recombinant human ER mannosidase I is absent from mock transfected cells but detected as a single immunoreactive 79 kDa band following transient expression Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.209 additional information Homo sapiens targets misfolded glycoproteins for dislocation into the cytosol and destruction by 26 S proteasomes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.209 Homo sapiens
-
-
-
3.2.1.209 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.209 additional information targets misfolded glycoproteins for dislocation into the cytosol and destruction by 26 S proteasomes Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.209 ER mannosidase I
-
 Homo sapiens
3.2.1.209 ER mannosidase I short-lived intracellular protein in mouse Mus musculus