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Literature summary extracted from

  • Gruber, C.W.; Cemazar, M.; Clark, R.J.; Horibe, T.; Renda, R.F.; Anderson, M.A.; Craik, D.J.
    A novel plant protein-disulfide isomerase involved in the oxidative folding of cystine knot defense proteins (2007), J. Biol. Chem., 282, 20435-20446.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.4.1 gene pdi, DNA and amino acid sequence determination and analysis, His-tagged PDI is co-expressed with the cyclotide precursor protein Oak1 in Escherichia coli strain BL21(DE3) using the pREP4 helper plasmid Oldenlandia affinis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.4.1 additional information
-
 additional information folding kinetics of linear and cyclic kalata B1 Oldenlandia affinis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.4.1 55000
-
 x * 55000, recombinant His-tagged enzyme, SDS-PAGE Oldenlandia affinis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.4.1 additional information Oldenlandia affinis the enzyme is involved in the oxidative folding of cystine knot defense proteins and in in the biosynthesis of insecticidal cyclotides, overview, the Oldenlandia affinis plant accumulates knotted circular proteins called cyclotides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.4.1 Oldenlandia affinis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.4.1 recombinant His-tagged PDI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Oldenlandia affinis

Reaction

EC Number Reaction Comment Organism Reaction ID
5.3.4.1 catalyses the rearrangement of -S-S- bonds in proteins mechanism of enzyme-assisted folding of plant cyclotides Oldenlandia affinis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.4.1 kalata B1 and derivatives, PDI dramatically enhanced the correct oxidative folding of linear and cyclic kalata B1 at physiological pH, determination of folding intermediates Oldenlandia affinis ?
-
 ?
5.3.4.1 additional information the enzyme is involved in the oxidative folding of cystine knot defense proteins and in in the biosynthesis of insecticidal cyclotides, overview, the Oldenlandia affinis plant accumulates knotted circular proteins called cyclotides Oldenlandia affinis ?
-
 ?
5.3.4.1 additional information PDI interacts with the cyclotide precursor protein Oak1, PDi is a functional oxidoreductase and exhibits both protein disulfide isomerase and chaperone activity, properties of disulfide species, overview Oldenlandia affinis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.4.1 ? x * 55000, recombinant His-tagged enzyme, SDS-PAGE Oldenlandia affinis
5.3.4.1 More primary and secondary PDi structure analysis, overview Oldenlandia affinis

Synonyms

EC Number Synonyms Comment Organism
5.3.4.1 PDI
-
 Oldenlandia affinis
5.3.4.1 protein-disulfide isomerase
-
 Oldenlandia affinis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.4.1 25
-
 assay at Oldenlandia affinis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.4.1 7.5
-
 assay at Oldenlandia affinis

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.4.1 GSH for oxidative folding Oldenlandia affinis
5.3.4.1 GSSG for reduction activity Oldenlandia affinis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.4.1 Oldenlandia affinis PDI C-terminal extension domain, isoelectric focusing
-
 6.6