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Literature summary extracted from

  • Simanshu, D.K.; Savithri, H.S.; Murthy, M.R.
    Crystal structures of Salmonella typhimurium biodegradative threonine deaminase and its complex with CMP provide structural insights into ligand-induced oligomerization and enzyme activation (2006), J. Biol. Chem., 281, 39630-39641.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.19 AMP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.19 CMP
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.19 into the pRSETC vector for expression in Escherichia coli BL21DE3 pLysS cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.19 data for two crystal forms are collected to resolutions of 2.2 and 1.7 A, crystals obtainted in the presence of CMP diffract to a resolution of 3-3.5 A, in the presence of AMP poorly Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.19 16
-
 L-threonine in the presence of 5 mM AMP Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.19 32
-
 L-threonine in the presence of 5 mM CMP Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.19 123
-
 L-threonine
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.19 45000
-
 determined by gel-filtration in the absence of CMP and AMP Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.19 100000
-
 determined by gel-filtration in the presence of CMP Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.19 120000
-
 determined by gel-filtration in the presence of AMP Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.19 L-threonine Salmonella enterica subsp. enterica serovar Typhimurium
-
 2-oxobutanoate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.19 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.19 using a nickel-nitrilotriacetic acid affinity column Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.19 L-threonine
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-oxobutanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.19 dimer native structure Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.19 tetramer in the complex with CMP Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
4.3.1.19 Threonine deaminase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.19 25
-
 activity assay Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.19 8
-
 activity assay Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.19 pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium