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Literature summary extracted from

  • Neves, A.R.; Pool, W.A.; Castro, R.; Mingote, A.; Santos, F.; Kok, J.; Kuipers, O.P.; Santos, H.
    The alpha-phosphoglucomutase of Lactococcus lactis is unrelated to the alpha-D-phosphohexomutase superfamily and is encoded by the essential gene pgmH (2006), J. Biol. Chem., 281, 36864-36873.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.2 glucose 1,6-bisphosphate absolutely required Lactococcus lactis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.2
-
 Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
5.4.2.2 additional information overproduction of enzyme leads to increased glycolytic flux and growth rate on galactose. Growth of a conditional knock-out strain is severely impaired when enzyme activity is below the control level Lactococcus lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.2.2 Cd2+
-
 Lactococcus lactis
5.4.2.2 D-fructose 1,6-bisphosphate
-
 Lactococcus lactis
5.4.2.2 Zn2+
-
 Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.2 0.0168
-
 alpha-D-glucose 1-phosphate pH 7.2, 30°C Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.2 Mg2+ required, less than 2% of activity in absence of Mg2+ Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.2.2 28000
-
 3 * 28276, calculated, 3 * 28000, SDS-PAGE Lactococcus lactis
5.4.2.2 28276
-
 3 * 28276, calculated, 3 * 28000, SDS-PAGE Lactococcus lactis
5.4.2.2 84300
-
 gel filtration Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Lactococcus lactis Q00G41
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 both native and recombinant enzyme Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate enzyme is highly specific for substrate and product Lactococcus lactis alpha-D-glucose 6-phosphate
-
 r
5.4.2.2 alpha-D-glucose 6-phosphate 23% of the activity with alpha-D-glucose 1-phosphate Lactococcus lactis alpha-D-glucose 1-phosphate
-
 r
5.4.2.2 additional information no substrate: alpha-D-mannose 1-phosphate, beta-D-glucose 1-phosphate Lactococcus lactis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.2.2 trimer 3 * 28276, calculated, 3 * 28000, SDS-PAGE Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.2 5.6 7.5 more than 50% of maximum activity within this range Lactococcus lactis
5.4.2.2 6.5
-
-
 Lactococcus lactis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.4.2.2 48
-
 D-fructose 1,6-bisphosphate pH 7.2, 30°C Lactococcus lactis