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Literature summary extracted from

  • Gonzalez, C.F.; Ackerley, D.F.; Lynch, S.V.; Matin, A.
    ChrR, a soluble quinone reductase of Pseudomonas putida that defends against H2O2. (2005), J. Biol. Chem., 280, 22590-22595.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.5.2 additional information about 10fold induction of enzyme expression by H2O2 Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.5.2 gene chrR, quantitative expression analysis and profile Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.6.5.2 additional information construction of ChrR overexpressing and knockout mutant strains showing increased and abolished tolerance of H2O2, respectively, enhancing the activity of ChrR in a chromate-remediating bacterial strain may not only increase the rate of chromate transformation, it may also augment the capacity of these cells to withstand the unavoidable production of H2O2 that accompanies chromate reduction, overview Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 additional information
-
 additional information steady-state kinetic analysis of the quinone reductase activity Pseudomonas putida
1.6.5.2 0.11
-
 benzoquinone pH 7.5, 70°C Pseudomonas putida
1.6.5.2 0.12
-
 coenzyme Q1 pH 8.5, 60°C Pseudomonas putida
1.6.5.2 0.21
-
 duroquinone pH 8.0, 70°C Pseudomonas putida
1.6.5.2 0.23
-
 menadione pH 8.0, 60°C Pseudomonas putida
1.6.5.2 0.26
-
 chromate(IV) pH 5.0, 70°C Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.2 soluble
-
 Pseudomonas putida
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.2 additional information Pseudomonas putida ChrR minimizes intracellular H2O2 stress ?
-
 ?
1.6.5.2 additional information Pseudomonas putida KT 2240 ChrR minimizes intracellular H2O2 stress ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Pseudomonas putida
-
 gene chrR
-
1.6.5.2 Pseudomonas putida KT 2240
-
 gene chrR
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.5.2 NAD(P)H + H+ + a quinone = NAD(P)+ + a hydroquinone reaction mechanism Pseudomonas putida
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.2 cell culture H2O2-scavenging and growth of wild-type, overexpressing, and knockout cells, overview Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 benzoquinone + NADH
-
 Pseudomonas putida ?
-
 ?
1.6.5.2 benzoquinone + NADH
-
 Pseudomonas putida KT 2240 ?
-
 ?
1.6.5.2 chromate(IV) + H2O
-
 Pseudomonas putida chromate(III) + H2O2
-
 ?
1.6.5.2 chromate(IV) + H2O
-
 Pseudomonas putida KT 2240 chromate(III) + H2O2
-
 ?
1.6.5.2 coenzyme Q1 + NADH
-
 Pseudomonas putida ?
-
 ?
1.6.5.2 duroquinone + NADH
-
 Pseudomonas putida ?
-
 ?
1.6.5.2 menadione + NADH
-
 Pseudomonas putida ?
-
 ?
1.6.5.2 menadione + NADH
-
 Pseudomonas putida KT 2240 ?
-
 ?
1.6.5.2 additional information ChrR minimizes intracellular H2O2 stress Pseudomonas putida ?
-
 ?
1.6.5.2 additional information ChrR reduces quinones by simultaneous two-electron transfer, avoiding formation of highly reactive semiquinone intermediates and producing quinols that promote tolerance of H2O2 Pseudomonas putida ?
-
 ?
1.6.5.2 additional information ChrR minimizes intracellular H2O2 stress Pseudomonas putida KT 2240 ?
-
 ?
1.6.5.2 additional information ChrR reduces quinones by simultaneous two-electron transfer, avoiding formation of highly reactive semiquinone intermediates and producing quinols that promote tolerance of H2O2 Pseudomonas putida KT 2240 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.5.2 chromate reductase
-
 Pseudomonas putida
1.6.5.2 ChrR
-
 Pseudomonas putida
1.6.5.2 Quinone reductase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.6.5.2 60 70
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.5.2 5.8
-
 chromate(IV) pH 5.0, 70°C Pseudomonas putida
1.6.5.2 6.4
-
 coenzyme Q1 pH 8.5, 60°C Pseudomonas putida
1.6.5.2 25
-
 duroquinone pH 8.0, 70°C Pseudomonas putida
1.6.5.2 440
-
 menadione pH 8.0, 60°C Pseudomonas putida
1.6.5.2 930
-
 benzoquinone pH 7.5, 70°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.2 5
-
 chromate reductase activity Pseudomonas putida
1.6.5.2 7.5 8.5 quinone reductase activity Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 flavin flavoenzyme Pseudomonas putida
1.6.5.2 NADH
-
 Pseudomonas putida