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Literature summary extracted from

  • Kahnert, A.; Kertesz, M.A.
    Characterization of a sulfur-regulated oxygenative alkylsulfatase from Pseudomonas putida S-313 (2000), J. Biol. Chem., 275, 31661-31667.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.17 2-oxoglutarate required for activity Pseudomonas putida
1.14.11.17 ascorbate 3fold increase of activity at 0.2 mM Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.17 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.17 EDTA 0.1 mM EDTA abolishes activity completely Pseudomonas putida
1.14.11.17 additional information no reduction in enzyme activity is observed for sulfate concentrations up to 10 mM Pseudomonas putida
1.14.11.17 nonyl sulfate inhibits at concentrations between 1 and 10 mM Pseudomonas putida
1.14.11.17 SDS inhibits at concentrations between 1 and 10 mM Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.17 Fe2+ required for activity, highest activity in the presence of 0.1 mM Pseudomonas putida
1.14.11.17 additional information Ni2+, Co2+, Mn2+, Cu2+, Zn2+, Mg2+, and Ca2+ have no stimulatory effect Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.11.17 30250
-
 4 * 30250, gel filtration Pseudomonas putida
1.14.11.17 32000
-
 4 * 32000, SDS-PAGE Pseudomonas putida
1.14.11.17 121000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.17 Pseudomonas putida
-
-
-
1.14.11.17 Pseudomonas putida S-313
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.17 Resource-Q anion-exchange column chromatography and Superdex 200 gel filtration Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.17 hexyl sulfate + 2-oxoglutarate + O2
-
 Pseudomonas putida hexanal + sulfite + succinate + CO2
-
 ?
1.14.11.17 hexyl sulfate + 2-oxoglutarate + O2
-
 Pseudomonas putida S-313 hexanal + sulfite + succinate + CO2
-
 ?
1.14.11.17 additional information the AtsK enzyme is not involved in the utilization of taurine as a sulfur source Pseudomonas putida ?
-
 ?
1.14.11.17 additional information the AtsK enzyme is not involved in the utilization of taurine as a sulfur source Pseudomonas putida S-313 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.17 homotetramer 4 * 32000, SDS-PAGE Pseudomonas putida
1.14.11.17 homotetramer 4 * 30250, gel filtration Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.11.17 alpha-ketoglutarate-dependent dioxygenase
-
 Pseudomonas putida
1.14.11.17 AtsK
-
 Pseudomonas putida
1.14.11.17 oxygenative alkylsulfatase
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.17 7
-
 in Tris-acetate buffer Pseudomonas putida