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Literature summary extracted from

  • Attieh, J.M.; Hanson, A.D.; Saini, H.S.
    Purification and characterization of a novel methyltransferase responsible for biosynthesis of halomethanes and methanethiol in Brassica oleracea (1995), J. Biol. Chem., 270, 9250-9257.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.165 bisulfide
-
 Brassica oleracea
2.1.1.165 S-adenosyl-L-homocysteine competitive with respect to S-adenosyl-L-methionine, noncompetitive to iodide Brassica oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.165 0.03
-
 S-adenosyl-L-methionine pH 7.5, 22°C, cosubstrate: iodide Brassica oleracea
2.1.1.165 1.3
-
 Iodide pH 7.5, 22°C Brassica oleracea
2.1.1.165 29
-
 bromide pH 7.5, 22°C Brassica oleracea
2.1.1.165 85
-
 chloride pH 7.5, 22°C Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.165 28000
-
 1 * 28000, SDS-PAFE Brassica oleracea
2.1.1.165 29500
-
 gel filtration Brassica oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.165 Brassica oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.165 gel filtration, anion exchange chromatography, and affinity chromatography on adenosine-agarose Brassica oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.165 leaf
-
 Brassica oleracea
-

Storage Stability

EC Number Storage Stability Organism
2.1.1.165 - 80°C, enzyme after the first gel filtration purification step, in 25 mM Tris acetate, pH 7.4, 10% glycerol, and 14 mM 2-mercaptoethanol, stable for over 2 months Brassica oleracea
2.1.1.165 -80°C, after affinity chromatography, the halide/bisulfide methyltransferase becomes extremely labile losing all activity after overnight storage Brassica oleracea
2.1.1.165 20°C, enzyme after the affinity chromatography purification step, 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 30% glycerol, 12% remaining activity after 48 h Brassica oleracea
2.1.1.165 4°C, enzyme after anion exchange purification step, in 25 mM Tris acetate, pH 7.4, 14 mM 2-mercaptoethanol, and 175 mM NaCl, more than 70% remaining activity after 24 h and 55% after 48 h Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.165 additional information the bifunctional enzyme also shows activity of EC 2.1.1.9 Brassica oleracea ?
-
 ?
2.1.1.165 S-adenosyl-L-methionine + bromide kcat/KM for bromide is 12529fold lower than kcat/Km for iodide Brassica oleracea S-adenosyl-L-homocysteine + methyl bromide
-
 ?
2.1.1.165 S-adenosyl-L-methionine + chloride kcat/KM for chloride is 19065fold lower than kcat/Km for iodide Brassica oleracea S-adenosyl-L-homocysteine + methyl chloride
-
 ?
2.1.1.165 S-adenosyl-L-methionine + iodide iodide is the preferred substrate Brassica oleracea S-adenosyl-L-homocysteine + methyl iodide
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.165 monomer 1 * 28000, SDS-PAFE Brassica oleracea

Synonyms

EC Number Synonyms Comment Organism
2.1.1.165 halide/bisulfide methyltransferase bifunctional enzyme also shows activity of EC 2.1.1.9 Brassica oleracea
2.1.1.165 S-adenosyl-L-methionine:halide/bisulfide methyltransferase bifunctional enzyme also shows activity of EC 2.1.1.9 Brassica oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.165 22
-
 assay at Brassica oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.165 5.5 7 methylation of iodide Brassica oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.165 0.032
-
 S-adenosyl-L-homocysteine pH 7.5, 22°C Brassica oleracea
2.1.1.165 1.35
-
 bisulfide pH 7.5, 22°C Brassica oleracea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.165 Brassica oleracea chromatofocusing
-
 4.8