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Literature summary extracted from

  • Mandal, P.; Chakraborty, P.; Sau, S.; Mandal, N.C.
    Purification and characterization of a deoxyriboendonuclease from Mycobacterium smegmatis (2006), J. Biochem. Mol. Biol., 39, 140-144.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.21.6 additional information deoxyribonuclease is an endonuclease, but is not a restriction endonuclease Mycolicibacterium smegmatis

General Stability

EC Number General Stability Organism
3.1.30.2 addition of 10 mM EDTA essential for storage stability Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.6 EDTA chelates Mg2+, increasing Mg2+ concentrations can restore the activity of the enzyme Mycolicibacterium smegmatis
3.1.21.6 K+
-
Mycolicibacterium smegmatis
3.1.21.6 additional information not inhibited by Na+ Mycolicibacterium smegmatis
3.1.30.2 EDTA 90% inhibition at 2.5 mM, inhibition fully reversible by addition of Mg2+ Mycolicibacterium smegmatis
3.1.30.2 K+
-
Mycolicibacterium smegmatis
3.1.30.2 additional information no inhibition by Na+ Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.6 Mg2+ required for activity, maximum activity at 2.5 mM Mycolicibacterium smegmatis
3.1.21.6 Mn2+ can replace Mg2+ showing better activity Mycolicibacterium smegmatis
3.1.30.2 Mg2+ optimum activity in the presence of 2.5 mM Mycolicibacterium smegmatis
3.1.30.2 Mn2+ can replace Mg2+, more effective than Mg2+ Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.6 43000
-
gel filtration Mycolicibacterium smegmatis
3.1.30.2 43000
-
x * 43000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.2 dsDNA + H2O Mycolicibacterium smegmatis
-
? DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends ?
3.1.30.2 dsDNA + H2O Mycolicibacterium smegmatis physiological function currently unknown ? DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, random DNA cleavage with respect to all possible phosphodiester bonds ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.6 Mycolicibacterium smegmatis
-
mc26 culture
-
3.1.30.2 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.6 to near homogeneity, 521fold by gel filtration Mycolicibacterium smegmatis
3.1.30.2 purified to near homogeneity Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.21.6 18
-
crude extract Mycolicibacterium smegmatis
3.1.21.6 9444
-
521fold purified enzyme Mycolicibacterium smegmatis
3.1.30.2 0.315
-
purified enzyme Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
3.1.21.6 0°C, Tris-HCl buffer, pH 7.4, 10 mM EDTA, 10% glycerol, stable for at least 15 days Mycolicibacterium smegmatis
3.1.30.2 0°C, Tris-HCl buffer, pH 7.4, 10 mM EDTA, 10% glycerol, 15 days storage on ice, fully stable for at least 15 days Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.6 covalently closed circular DNA pUC18 + H2O
-
Mycolicibacterium smegmatis ?
-
?
3.1.21.6 lambda DNA + H2O
-
Mycolicibacterium smegmatis ?
-
?
3.1.21.6 additional information catalyzes the endonucleolytic cleavage of both the double- as well as the single-strand DNA non-specifically Mycolicibacterium smegmatis ?
-
?
3.1.21.6 pBR322 DNA + H2O
-
Mycolicibacterium smegmatis DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, respectively
-
?
3.1.30.2 DNA + H2O tritium labeled DNA from Escherichia coli, fragmented by limited sonication Mycolicibacterium smegmatis ? random DNA cleavage with respect to all possible phosphodiester bonds ?
3.1.30.2 dsDNA + H2O
-
Mycolicibacterium smegmatis ? DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends ?
3.1.30.2 dsDNA + H2O physiological function currently unknown Mycolicibacterium smegmatis ? DNA fragments carrying phosphate groups at 5' ends and hydroxyl group at the 3' ends, random DNA cleavage with respect to all possible phosphodiester bonds ?
3.1.30.2 lambda DNA + H2O
-
Mycolicibacterium smegmatis ? size of linear DNA fragments decreases with prolonged incubation time ?
3.1.30.2 pUC18 DNA + H2O covalently closed circular plasmid DNA Mycolicibacterium smegmatis ? substrate converted into relaxed circular form and than to the linear form ?

Subunits

EC Number Subunits Comment Organism
3.1.30.2 ? x * 43000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
3.1.21.6 Deoxyriboendonuclease
-
Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.6 32
-
-
Mycolicibacterium smegmatis
3.1.30.2 32
-
-
Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.6 7.2
-
-
Mycolicibacterium smegmatis
3.1.30.2 7.2
-
-
Mycolicibacterium smegmatis