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Literature summary extracted from

  • Zhao, H.; Charnley, A.K.; Wang, Z.; Yin, Y.; Li, Z.; Li, Y.; Cao, Y.; Peng, G.; Xia, Y.
    Identification of an extracellular acid trehalase and its gene involved in fungal pathogenesis of Metarhizium anisopliae (2006), J. Biochem., 140, 319-327.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 trehazolin
-
 Metarhizium anisopliae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 2.3
-
 trehalose in 20 mM MES buffer, pH 5.5, at 30°C Metarhizium anisopliae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 114300
-
 calculated from amino acid sequence Metarhizium anisopliae
3.2.1.28 130000
-
 SDS-PAGE, deglycosylated enzyme Metarhizium anisopliae
3.2.1.28 170000
-
 SDS-PAGE Metarhizium anisopliae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Metarhizium anisopliae A9XE63 fragment; subspecies Metarhizium anisopliae acridum, strain CQMa102
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.28 glycoprotein 23.5% carbohydrate content Metarhizium anisopliae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28 High Q Sepharose column chromatography, 25Q Sepharose column chromatography, and Sephacryl-200s HR gel filtration Metarhizium anisopliae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 1.224
-
 culture filtrate, in 20 mM MES buffer, pH 5.5, at 30°C Metarhizium anisopliae
3.2.1.28 120
-
 after 98.04fold purification, in 20 mM MES buffer, pH 5.5, at 30°C Metarhizium anisopliae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 additional information does not hydrolyze cellobiose, maltose, sucrose, lactose, p-nitrophenyl galactopyranoside, p-nitrophenyl alpha-glucopyranoside, or p-nitrophenyl beta-glucopyranoside Metarhizium anisopliae ?
-
 ?
3.2.1.28 trehalose + H2O highly specific for trehalose Metarhizium anisopliae D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.28 acid trehalase
-
 Metarhizium anisopliae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 30
-
-
 Metarhizium anisopliae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.28 20 45
-
 Metarhizium anisopliae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 55
-
 activity decreases rapidly at 55°C or above Metarhizium anisopliae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 5.5
-
-
 Metarhizium anisopliae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.28 4.5 7
-
 Metarhizium anisopliae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.28 Metarhizium anisopliae isoelectric focusing
-
 4.7

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.28 0.000013
-
 in 20 mM MES buffer, pH 5.5, at 30°C Metarhizium anisopliae trehazolin