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Literature summary extracted from

  • Kim, S.; Lee, S.B.
    Catalytic promiscuity in dihydroxy-acid dehydratase from the thermoacidophilic archaeon Sulfolobus solfataricus (2006), J. Biochem., 139, 591-596.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.9 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.9 2-mercaptoethanol 1 mM, 40% inhibition Saccharolobus solfataricus
4.2.1.9 Ca2+
-
 Saccharolobus solfataricus
4.2.1.9 Co2+
-
 Saccharolobus solfataricus
4.2.1.9 Cu2+
-
 Saccharolobus solfataricus
4.2.1.9 Fe2+
-
 Saccharolobus solfataricus
4.2.1.9 Mg2+
-
 Saccharolobus solfataricus
4.2.1.9 p-chloromercuribenzoic acid
-
 Saccharolobus solfataricus
4.2.1.9 p-hydroxymercuribenzoic acid
-
 Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.9 0.54
-
 2,3-dihydroxyisovalerate
-
 Saccharolobus solfataricus
4.2.1.9 2.42
-
 D-gluconate
-
 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.9 Mn2+ enhances activity Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.9 12000
-
 gel filtration Saccharolobus solfataricus
4.2.1.9 66000
-
 2 * 66000, SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.9 additional information Saccharolobus solfataricus dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids ?
-
 ?
4.2.1.9 additional information Saccharolobus solfataricus P2 dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.9 Saccharolobus solfataricus Q97UB2
-
-
4.2.1.9 Saccharolobus solfataricus P2 Q97UB2
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.9 when the recombinant enzyme is incubated in 50 mM Tris-HCl buffer at 30°C for 2 h under aerobic or anaerobic conditions and the residual activity is measured, no difference is found between aerobic and anaerobic conditions Saccharolobus solfataricus
4.2.1.9 when the recombinant enzyme is incubated in 50 mM Tris-HCl buffer at 30°C for 2 h with organic peroxide, the enzyme activity is stable during exposure to 0.5% organic peroxide for 2 h Saccharolobus solfataricus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.9
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.9 2,3-dihydroxyisovalerate
-
 Saccharolobus solfataricus ?
-
 ?
4.2.1.9 D-arabonate
-
 Saccharolobus solfataricus ?
-
 ?
4.2.1.9 D-arabonate
-
 Saccharolobus solfataricus P2 ?
-
 ?
4.2.1.9 D-fuconate
-
 Saccharolobus solfataricus ?
-
 ?
4.2.1.9 D-fuconate
-
 Saccharolobus solfataricus P2 ?
-
 ?
4.2.1.9 D-Galacturonate
-
 Saccharolobus solfataricus ?
-
 ?
4.2.1.9 D-gluconate
-
 Saccharolobus solfataricus 2-keto-3-deoxygluconate + H2O
-
 ?
4.2.1.9 D-Glucuronate
-
 Saccharolobus solfataricus ?
-
 ?
4.2.1.9 D-Glucuronate
-
 Saccharolobus solfataricus P2 ?
-
 ?
4.2.1.9 D-xylonate
-
 Saccharolobus solfataricus ?
-
 ?
4.2.1.9 D-xylonate
-
 Saccharolobus solfataricus P2 ?
-
 ?
4.2.1.9 additional information dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids Saccharolobus solfataricus ?
-
 ?
4.2.1.9 additional information dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids Saccharolobus solfataricus P2 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.9 dimer 2 * 66000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.9 80
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.9 60 90 60°C: about 60% of maximal activity, 90°C: about 60% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.9 70
-
 2 h, stable Saccharolobus solfataricus
4.2.1.9 80
-
 30 min, 50% loss of activity Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.9 48.5
-
 D-gluconate
-
 Saccharolobus solfataricus
4.2.1.9 75.8
-
 2,3-dihydroxyisovalerate
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.9 7.5 8.5
-
 Saccharolobus solfataricus